Standard SPE Procedure for CHROMABOND® PS-Mix

Method:	Matrix	Application-No.:
SPE	-	304037

Substances:
Column:		CHROMABOND® PS-Mix, 3 ml, 200 mg
Phase:		CHROMABOND PS-Mix
MN catalog number:		on request
Sample pretreatment:		Individual sample preparation in reference to the compounds and matrix.
Conditions:		Column conditioning: 3 ml methanol, then 3 ml water. (Do not let run the column dry.) Sample aspiration: The prepared sample is passed through the column by vacuum or pressure (max. 1000 ml sample volume) Washing : With 3 ml AcOH / water (5:95, v/v) impurities are removed. Elution 1: Polar and basic compounds are eluated with 3 ml methanol / water (1:1, v/v) + 10% NH3 Elution 2: Polar and acidic compounds are eluated with 3 ml methanol / water (1:1, v/v) + 10% AcOH Elution 3: Nonpolar and neutral compounds are eluated with 3 ml methanol / acetone (1:5, v/v)
Detection Subsequent analysis:
Author(-s):		SPE department
Source:		MACHEREY-NAGEL
Keywords:		standard SPE procedure
Chromatogram:
Legend:		All solvent volumes are based on the amount of adsorbent (200 or 500 mg) and have to be adjusted according to the size of used cartridge. This protocol is a suggestion for standard procedures and should be optimized (solvents, volumes, adsorbent amount, ....) due to necessity of the investigated sample or compound and subsequent analysis (GC, HPLC, ...).

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