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| Substances: |
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naphthalene; acenaphthylene; acenaphthene; fluorene; phenanthrene; anthracene; fluoranthene; pyrene; benzanthracene; chrysene; benzofluoranthene; benzopyrene; dibenzanthracene; benzoperylene; indenopyrene |
| Column: |
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CHROMABOND® CN / SiOH, 6 ml, 500/1000 mg |
| Phase: |
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Chromabond CN/SiOH |
| MN catalog number: |
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730135 |
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| Sample pretreatment: |
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Dry 30 g soil with sodium sulphate and reflux 4 hours with 250 ml petroleum
ether in a Soxhlet extractor. For low PAH contents (colorless or weakly colored
extracts) concentrate extract to 1/10 of its volume in a rotation evaporator. |
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| Conditions: |
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Column conditioning:
4 ml petroleum ether
Sample application:
Force or aspirate 20 ml of the extract through the column
Column washing:
2 ml petroleum ether
Elution:
2 x 2 ml acetonitrile / benzene (3:1, v/v), then evaporate or fill up to the
volume required (alternative 2 x 2 ml acetonitrile / toluene (3:1, v/v))
For further analysis we recommend HPLC e.g. with column 250/3 NUCLEOSIL® 5
C18 PAH |
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Detection
Subsequent analysis: |
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| Author(-s): |
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Macherey-Nagel |
| Source: |
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LC Department |
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| Keywords: |
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pah; soil |
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Chromatogram:
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| Legend: |
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Compound recovery [%]
Naphthalene 85
Acenaphthylene 92
Acenaphthene 89
Fluorene 87
Phenanthrene 83
Anthracene 88
Fluoranthene 87
Pyrene 90
Benz[a]anthracene 84
Chrysene 96
Benzo[b]fluoranthene 95
Benzo[k]fluoranthene 90
Benzo[a]pyrene 90
Dibenz[ah]anthracene 96
Benzo[ghi]perylene 87
Indeno[1,2,3-cd]pyrene 97 |