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| Ion exchange analysis of synthetic oligonucleotides |
Method: | Matrix | Application-No.: | HPLC | - | 107360 |
| | | | Substances: | | oligonucleotide | | Column: | | VA50/4.6 NUCLEOGEL® SAX 1000-8 | | Phase: | | NUCLEOGEL SAX | | MN catalog number: | | | | | | | | Sample pretreatment: | | Ion exchange analysis of synthetic oligonucleotides | | | | | | Conditions: | | eluent A: 0.02M KH2PO4 pH 5.5, 5M urea; eluent B: A + 1M KCl, linear gradient 0 - 100% B in 30 min
flow rate 1ml/min | | | | | Detection
Subsequent analysis: | | UV, 260nm | | | |
| | Author(-s): | | MACHEREY-NAGEL | | Source: | | LC department | | | |
| | Keywords: | | nucleic acids; nucleotides | | | | | | | | Chromatogram:
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| | | | | | Legend: | | Separation of synthetic oligonucleotides: 1. 6mer, 2. 24mer, 3. 30mer, 4. 29mer |
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