Standard SPE Procedure for CHROMABOND® C18 ec

Method:	Matrix	Application-No.:
SPE	-	304031

Substances:
Column:		CHROMABOND® C18 ec, 6 ml, 500 mg
Phase:		CHROMABOND C18 ec
MN catalog number:		730014
Sample pretreatment:		Individual sample preparation in reference to the compounds and matrix.
Conditions:		Column conditioning: 6 ml methanol, then 6 ml water. (Do not let run the column dry.) Sample aspiration: The prepared sample is passed through the column by vacuum or pressure (max. 1000 ml sample volume) Washing: With 3 ml methanol - water (5:95, v/v) are washed impurities Elution: Analytes are eluted with 2 x 2 ml methanol or acetone
Detection Subsequent analysis:
Author(-s):		SPE department
Source:		MACHEREY-NAGEL
Keywords:		standard SPE procedure
Chromatogram:
Legend:		All solvent volumes are based on the amount of adsorbent (200 or 500 mg) and have to be adjusted according to the size of used cartridge. This protocol is a suggestion for standard procedures and should be optimized (solvents, volumes, adsorbent amount, ....) due to necessity of the investigated sample or compound and subsequent analysis (GC, HPLC, ...).

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