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| Rapid Determination of Heavy Polycyclic Aromatic Hydrocarbons in Edible Fats and Oils |
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Method:
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Matrix
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Application-No.:
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SPE
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edible oil, fat
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303060
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| Substances: |
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6-nuclear PAHs; 5-nuclear PAHs; 4-nuclear PAHs; 3-nuclear PAHs; 2-nuclear PAHs; benzo[a]anthracene; benzo[b]chrysene |
| Column: |
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CHROMABOND® HR-P, 6 mL, 500 mg (glass columns) |
| Phase: |
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CHROMABOND® HR-P |
| REF number: |
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730111G |
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| Sample pretreatment: |
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In a small beaker 2.0 - 2.5 g of sample were dissolved in 5 mL isohexane / t-butyldimethylether (95:5, v/v). 50 μL internal standard solution (benzo[b]chrysene,
1 ng/μL isooctane) were added. |
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| Conditions: |
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Column conditioning: with 10 mL isohexane / t-butyldimethylether (95:5, v/v)
Sample application: The solution was transferred onto the cartridge and the beaker rinsed with additional 2 mL isohexane / t-butyldimethylether (95:5, v/v).
Column washing: The cartridge was washed with 10 mL isohexane / t-butylmethylether (95:5, v/v), followed by a small volume of air (about 3 mL). The eluate was discarded afterwards. The cartridges was washed again with 10 mL isohexane / t-buthylmethylether (80:20, v/v), followed by a small volume of air (about 3 mL), the eluate discarded once more. Again the cartridges was washed with 10 mL isohexane / t-buthylmethylether (50:50, v/v) followed by a small volume of air (about 3 mL), the eluate once more discarded.
Elution: PAHs were eluted with 20 mL THF.
Evaporation: 50 μL acetonitrile / diethylenglycole (2:1, v/v) were added and the eluate evaporated to dryness. The residue was dissolved in 500 μL acetonitrile and transferred to an autosampler vial. |
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| Subsequent analysis: |
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HPLC, e.g with NUCLEODUR® C18 PAH
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| Author(-s): |
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Weißhaar, R. |
| Source: |
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Eur. J. Lipid Sci. Technol. 104 (2002) 282-285 |
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| Keywords: |
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PAHs; fat; oil |
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Recovery rates:
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Benzo(b)chrysene - 85-95 %
Benzo(a)anthracene - 70-75 %
6- and 5-nuclear PAHs - 85-95 %
4-nuclear PAHs - 60-75%
2- and 3-nuclear PAHs - <75 %
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| www.mn-net.com |
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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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