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Separation of pancreatic secretion of piglet

Method:
Matrix
Application-No.:
HPLC
pancreatic secretion
108280

Substances:trypsin; trypsinogen; proelastase; lipase; chymotrypsin-a; chymotrypsinogen; amylase-a; procarboxypeptidase
Column:NUCLEOSIL® 500-5C18 PPN, 125 x 4mm ID
Phase:Nucleosil 500-5 C18 PPN
MN catalog number:
Sample pretreatment:the following separation of catabolic enzymes from pancreas secretion.
Conditions:eluent A: 0.1% TFA in H2O, eluent B: 0.08% trifluoroacetic acid in CH3CN, gradient from 30 to 50% B in 14min, then from 50 to 65% B in 6min
flow rate 1ml/min
Detection
Subsequent analysis:
UV, 215nm

Author(-s):MACHEREY-NAGEL
Source:LC department

Keywords:proteins
Chromatogram:



Legend:Separation of pancreatic secretion of piglet, 1. trypsin + trypsinogen, 2. proelastase, 3. lipase + a-chymotrypsin, 4. chymotrypsinogen, 5. a-amylase, 6. and 7. procarboxypeptidase, sample volume 2μl.



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