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| Substances: |
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trypsin; trypsinogen; proelastase; lipase; alpha-chymotrypsin; chymotrypsinogen; alpha-amylase; procarboxypeptidase |
| Column: |
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EC 125/4 NUCLEOSIL® 500-5 C18 PPN |
| Phase: |
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NUCLEOSIL® C18 PPN |
| REF number: |
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720257.40
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| Conditions: |
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Eluent A: 0.1 % TFA in water
Eluent B: 0.08 % TFA in acetonitrile
Gradient: 30–50 % B in 14 min, 50–65 % B in 6 min
Flow rate: 1 mL/min
Injection volume: 2 μL
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| Detection: |
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UV, 215 nm |
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| Author(-s): |
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LC department |
| Source: |
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MACHEREY-NAGEL |
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| Keywords: |
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proteins; pancreatic secretion
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Chromatogram:
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| Legend: |
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Separation of pancreatic secretion of piglet
1. trypsin + trypsinogen
2. proelastase
3. lipase + alpha-chymotrypsin
4. chymotrypsinogen
5. alpha-amylase,
6. and 7. procarboxypeptidase |