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| Separation of pancreatic secretion of piglet |
Method: | Matrix | Application-No.: | HPLC | pancreatic secretion | 108280 |
| | | | Substances: | | trypsin; trypsinogen; proelastase; lipase; chymotrypsin-a; chymotrypsinogen; amylase-a; procarboxypeptidase | | Column: | | NUCLEOSIL® 500-5C18 PPN, 125 x 4mm ID | | Phase: | | Nucleosil 500-5 C18 PPN | | MN catalog number: | | | | | | | | Sample pretreatment: | | the following separation of catabolic enzymes from pancreas secretion. | | | | | | Conditions: | | eluent A: 0.1% TFA in H2O, eluent B: 0.08% trifluoroacetic acid in CH3CN, gradient from 30 to 50% B in 14min, then from 50 to 65% B in 6min
flow rate 1ml/min | | | | | Detection
Subsequent analysis: | | UV, 215nm | | | |
| | Author(-s): | | MACHEREY-NAGEL | | Source: | | LC department | | | |
| | Keywords: | | proteins | | | | | | | | Chromatogram:
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| | | | | | Legend: | | Separation of pancreatic secretion of piglet, 1. trypsin + trypsinogen, 2. proelastase, 3. lipase + a-chymotrypsin, 4. chymotrypsinogen, 5. a-amylase, 6. and 7. procarboxypeptidase, sample volume 2μl. |
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MACHEREY-NAGEL does not warrant the correctness of the applications. |
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