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| Enantioselective Microbial Degradation of Racemates Studied by Chiral HPLC on Silica-bound Albumin |
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Method:
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Matrix
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Application-No.:
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HPLC
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-
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105512
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| Substances: |
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d-benzoyl-n-alanine; l-benzoyl-n-alanine |
| Column: |
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EC 150/4 RESOLVOSIL® BSA-7 |
| Phase: |
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Resolvosil BSA-7 |
| MN catalog number: |
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720046.40 |
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| Sample pretreatment: |
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| Conditions: |
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eluent: 40mM phosphate buffer pH 7.05 + 1% 1-propanol
flow rate: 1.5ml/min |
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Detection
Subsequent analysis: |
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UV, 225nm, 0.16 AUFS |
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| Author(-s): |
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Allenmark, S. |
| Source: |
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Enzyme Microbiol. Technol. 8 (1986) 404 - 408 |
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| Keywords: |
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enantiomers; aromatic amino acids |
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Chromatogram:
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| Legend: |
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Hydrolysis of N-benzoyl-D,L-alanine (200μM) by Nocardia restrictus in 50mM phosphate buffer of pH 6.0 at 30°C. Direct analysis of samples from the reaction mixture withdrawn at certain time intervals.
Please see also: S. Allenmark, B. Bomgren and S. Andersson, Prep. Biochem. 14 (1984), 139 - 147. |
| www.mn-net.com |
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MACHEREY-NAGEL does not warrant the correctness of the applications. |
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