|
|
|
| Substances: |
|
PCB |
| Column: |
|
Kombi-Kit PCB from oil (DIN 51 527):
CHROMABOND® SiOH-H2SO4/SA, 3 mL, 500/500 mg,
CHROMABOND® SiOH, 3 mL, 500 mg
|
| Phase: |
|
CHROMABOND® SiOH-H2SO4/SA
CHROMABOND® SiOH |
| REF number: |
|
730125 |
|
|
|
| Sample pretreatment: |
|
Extract oil-contaminated solids with n-hexane. Homogenise other oil samples and dissolve 1.5 to 2.0 g in 50 mL n-hexane. Water which may cause turbidities can be removed with sodium sulphate. |
|
|
|
| Conditions: |
|
Determination with reference to German Standard DIN 51 527
Column conditioning:
Let 1 mL n-hexane flow through the CHROMABOND® SiOH-H2SO4/SA column.
Sample application:
Aspirate or force 500 μL sample through the CHROMABOND® SiOH-H2SO4/SA column. This phase offers better removal of interfering substances due to sulphonisation. Place CHROMABOND® SiOH-H2SO4/SA column on top of CHROMABOND® SiOH column with the aid of an adaptor (REF 730100) and after at least 30 seconds flush sample into the SiOH column with 2 x 1 mL n-hexane.
Elution:
Elute SiOH column with 3 x 0.5 mL n-hexane; adjust to a suitable concentration for subsequent GC analysis by evaporation of n-hexane in a stream of nitrogen or by dilution with n-hexane. |
|
|
|
|
|
|
|
|
|
| Author(-s): |
|
SPE department
|
| Source: |
|
MACHEREY-NAGEL |
|
|
|
| Keywords: |
|
PCB; oil; DIN 51 527; Kombi-Kit PCB |
|
|
|
|
|
|
|
|
|
|
|
|
|
| Recovery rates: |
|
Compound recovery [%]
PCB 28 99
PCB 52 95
PCB 101 99
PCB 138 94
PCB 153 99
PCB 180 96
PCB 209 101 |