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CHROMABOND® Diamino
CHROMABOND® QuEChERS |
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| Special silica phase for determination of pesticides in food samples |
| Features |
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Base material silica, pore size 60 Å, particle size 45 μm, specific surface 500 m2/g, pH stability 2–8
Primary and Secondary Amine functions (PSA), 5 % C
Removes polar compounds (e.g., organic acids, pigments, sugars) from matrices like fruit or vegetables
Similar phases: Supelclean PSA, Bond Elut PSA |
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Recommended application:
special SPE phase for quick and cheap determination of pesticides in strongly matrix-contaminated samples by GC-MS and LC-MS (see application 303770 and application 305060)
QuEChERS method = Quick Easy Cheap Effective Rugged Safe |
QuEChERS method and pre-mixes
Within a few years after its development by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food samples by GC-MS or LC-MS, allowing rapid and cheap clean-up of strongly matrix-contaminated samples.
For optimizing the extraction of pH-dependent compounds, for minimizing decomposition of sensitive substances, and for broadening the matrix spectrum, different modifications of the QuEChERS method have been elaborated.
In addition to the required adsorbent CHROMABOND® Diamino MACHEREY-NAGEL offers a number of individually weighed and premixed buffer and extraction mixtures, specially composed for different sample matrices.
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For extraction, the European standard EN 15662 recommends a citrate extraction mix (Mix I), while AOAC standard 2007.1 uses an acetate extraction mix (Mix II).
For clean-up, the Diamino phase (PSA) removes, e.g., sugars and organic acids. Magnesium sulfate removes water, C18 ec removes nonpolar interferences such as fats and the Carbon phase removes pigments, sterols, and nonpolar interferences.
For selection of the proper clean-up mix see table below.
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QuEChERS mixes
| Sample property |
Low fat content
(e.g., apples, strawberries) |
Moderate content of chlorophyll and carotinoids (e.g., carrots, lettuce) |
Higher content of chlorophyll and carotinoids (e.g., bell peppers, spinach) |
Higher fat content
(e.g., avocado) |
| CHROMABOND® QuEChERS extraction mixes |
| Citrate or acetate extraction |
Citrate or acetate extraction |
Citrate extraction |
Citrate extraction |
| Mix I or Mix II |
Mix I or Mix II |
Mix I |
Mix I |
| CHROMABOND® QuEChERS clean-up mixes |
| Diamino clean-up |
Diamino/Carbon clean-up |
Diamino/Carbon clean-up
(higher Carbon content) |
Diamino/C18 ec clean-up |
| Mix III |
Mix IV |
Mix V |
Mix VI |
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Ordering information
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Volume |
Description |
Composition |
REF |
Pack of |
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CHROMABOND® QuEChERS extraction buffer mixes |
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15 mL* |
Mix I |
citrate extraction mix |
4 g MgSO4, 1 g NaCl,
0.5 g Na2H citrate ∙ 1.5 H2O,
1 g Na3 citrate ∙ 2 H2O |
730970 |
50 |
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15 mL* |
Mix II |
acetate extraction mix |
6 g MgSO4, 1.5 g Na acetate |
730971 |
50 |
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CHROMABOND® QuEChERS clean-up mixes |
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15 mL* |
Mix III |
Diamino clean-up mix |
0.15 g CHROMABOND®
Diamino with 0.9 g MgSO4 |
730972 |
50 |
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15 mL* |
Mix IV |
Diamino/Carbon
clean-up mix |
0.15 g CHROMABOND®
Diamino with 0.9 g MgSO4
and 15 mg Carbon |
730973 |
50 |
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15 mL* |
Mix V |
Diamino/Carbon
clean-up mix |
0.15 g CHROMABOND®
Diamino with 0.9 g MgSO4
and 45 mg Carbon |
730975 |
50 |
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15 mL* |
Mix VI |
Diamino/C18 ec
clean-up mix |
0.15 g CHROMABOND®
Diamino with 0.9 g MgSO4
and 150 mg C18 ec |
730974 |
50 |
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CHROMABOND® Diamino polypropylene columns |
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3 mL |
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adsorbent weight 200 mg |
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730561 |
50 |
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6 mL |
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adsorbent weight 500 mg |
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730562 |
30 |
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CHROMABOND® Diamino adsorbent |
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730653.20 |
20 g |
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730653 |
100 g |
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CHROMABOND® QuEChERS accessories |
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50 mL polypropylene centrifuge tube with screw cap |
730223 |
50 |
| * 15 mL centrifuge tubes with screw cap (2 mL or 50 mL centrifuge tubes on request) |
| A number of custom-made QuEChERS mixes is available on request. |
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