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CHROMABOND® Diamino NEW!
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special silica phase for determination
of pesticides in food samples
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Features
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base material silica, pore size 60 Å, particle size 45 μm, specific surface 500 m2/g, pH stability 2 – 8
Primary and Secondary Amine functions (PSA), 5 % C
removes polar compounds (e. g. organic acids, pigments, sugars) from matrices like fruit or vegetables
similar phases: Supelclean PSA, Bond Elut PSA
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recommended application:
special SPE phase for quick and cheap determination of pesticides in strongly matrix-contaminated samples by GC (see application 303770)
QuEChERS method = Quick Easy Cheap Effective Rugged Safe
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QuEChERS method and pre-mixes
Within a few years after its development by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food samples by GC-MS or LC-MS, allowing rapid and cheap clean-up of strongly matrix-contaminated samples.
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Procedure 1 for standard food samples:
The sample is extracted with Mix II, then purified with Mix III or Mix IV (food with higher fat content)
Procedure 2 for complex or rich food samples:
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For optimising the extraction of pH-dependent compounds, for minimising decomposition of sensitive substances, and for broadening the matrix spectrum, different modifications of the QuEChERS method have been elaborated.
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The sample is extracted with Mix I, then purified with
Mix III (samples with low fat content),
Mix IV (moderate content of chlorophyll and carotinoids; e. g. carrots, lettuce),
Mix V (high content of chlorophyll and carotinoids; e. g. bell peppers, spinach) or
Mix VI (higher fat content; e. g. avocados)
For detailed instructions please visit the original references at www.quechers.com.
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In addition to the required adsorbent CHROMABOND® Diamino MACHEREY-NAGEL offers a number of individually weighed and premixed buffer and extraction mixtures, specially composed for different sample matrices.
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Ordering information
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Volume
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Description
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Composition
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Cat. No.
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Pack of
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| CHROMABOND® QuEChERS extraction buffer mixes
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15 ml*
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Mix I
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citrate extraction mix
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4 g MgSO4, 1 g NaCl,
0.5 g Na2H citrate ∙ 1.5 H2O,
1 g Na3 citrate ∙ 2 H2O
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730970
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50
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15 ml* |
Mix II
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acetate extraction mix
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6 g MgSO4, 1.5 g Na acetate
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730971
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50
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CHROMABOND® QuEChERS clean-up mixes containing 0.15 g CHROMABOND® Diamino each
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15 ml*
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Mix III
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Diamino clean-up mix
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with 0.9 g MgSO4
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730972
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50
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15 ml*
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Mix IV
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Diamino/Carbon clean-up mix
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with 0.9 g MgSO4 and
15 mg Carbon
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730973
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50
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15 ml*
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Mix V
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Diamino/Carbon clean-up mix |
with 0.9 g MgSO4 and
45 mg Carbon
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730975
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50
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15 ml*
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Mix VI
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Diamino/C18 ec clean-up mix |
with 0.9 g MgSO4 and
150 mg C18 ec
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730974
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50
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| CHROMABOND® Diamino polypropylene columns
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3 ml
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adsorbent weight 200 mg
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730561
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50
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6 ml
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adsorbent weight 500 mg
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730562
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30
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| CHROMABOND® Diamino adsorbent
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730653.20
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20 g
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730653
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100 g
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| CHROMABOND® QuEChERS accessories
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50 ml polypropylene centrifuge tube with screw cap
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730223
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50
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* 15 ml centrifuge tubes with screw cap
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