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CHROMABOND® Diamino
CHROMABOND® QuEChERS
Special silica phase for determination of pesticides in food samples
Features
Base material silica, pore size 60 Å, particle size 45 μm, specific surface 500 m2/g, pH stability 2–8
Primary and Secondary Amine functions (PSA), 5 % C
Removes polar compounds (e.g., organic acids, pigments, sugars) from matrices like fruit or vegetables
Similar phases: Supelclean PSA, Bond Elut PSA
Recommended application:
special SPE phase for quick and cheap determination of pesticides in strongly matrix-contaminated samples by GC-MS and LC-MS (see application 303770 and application 305060)
QuEChERS method = Quick Easy Cheap Effective Rugged Safe


QuEChERS method and pre-mixes
Within a few years after its development by Anastassiades et al. the QuEChERS method has gained a leading position for the determination of pesticide residues in food samples by GC-MS or LC-MS, allowing rapid and cheap clean-up of strongly matrix-contaminated samples.
For optimizing the extraction of pH-dependent compounds, for minimizing decomposition of sensitive substances, and for broadening the matrix spectrum, different modifications of the QuEChERS method have been elaborated.
In addition to the adsorbent CHROMABOND® Diamino MACHEREY-NAGEL offers a number of individually weighed and premixed extraction (buffered or non-buffered) and clean-up mixtures, specially composed for different sample matrices.
  For extraction, the European standard EN 15662 recommends a citrate extraction mix (Mix I), while the AOAC standard 2007.1 uses an acetate extraction mix (Mix II). A non-buffered extraction mix is used in the original method (Mix XII).
For clean-up, the Diamino phase (PSA) removes, e.g., sugars and organic acids. Magnesium sulfate removes water, C18 ec removes nonpolar interferences such as fats and the Carbon phase removes pigments, sterols, and nonpolar interferences.
For the selection of the proper clean-up mix see table below.

For more information please see our CHROMABOND® QuEChERS brochure.


QuEChERS mixes
Sample property
Low fat content
(e.g., apples, strawberries)
Moderate content of chlorophyll and carotinoids (e.g., carrots, lettuce) Higher content of chlorophyll and carotinoids (e.g., bell peppers, spinach) Higher fat content
(e.g., avocado)
CHROMABOND® QuEChERS extraction mixes
Citrate or acetate extraction Citrate or acetate extraction Citrate extraction Citrate extraction
Mix I or Mix II Mix I or Mix II Mix I Mix I
CHROMABOND® QuEChERS clean-up mixes
Diamino clean-up Diamino/Carbon clean-up Diamino/Carbon clean-up
(higher carbon content)
Diamino/C18 ec clean-up
Mix III or Mix XX Mix IV or Mix XVII Mix V Mix VI or Mix XIX


Ordering information
    Volume Description Composition REF Pack of
  CHROMABOND® QuEChERS extraction mixes
  15 mL* Mix I Citrate extraction mix
(EN 15662)
4000 mg MgSO4
1000 mg NaCl
500 mg Na2H citrate ∙ 1.5 H2O
1000 mg Na3 citrate ∙ 2 H2O
730970 50
  15 mL* Mix II Acetate extraction mix
(AOAC 2007.01)
6000 mg MgSO4
1500 mg Na acetate
730971 50
  15 mL*
Mix XII
Original extraction mix
4000 mg MgSO4
1000 mg NaCl
730648 50
 
CHROMABOND® QuEChERS clean-up mixes (EN 15662)
 
15 mL* Mix III Diamino clean-up mix 900 mg MgSO4
150 mg Diamino
730972 50
    2 mL* Mix III Diamino clean-up mix  150 mg MgSO4
25 mg Diamino
730646.2 50
 
15 mL* Mix IV Diamino/Carbon
clean-up mix
900 mg MgSO4
150 mg Diamino
15 mg Carbon
730973 50
    2 mL* Mix IV Diamino/Carbon
clean-up mix
150 mg MgSO4
25 mg Diamino
2.5 mg Carbon
730850.2 50
 
15 mL* Mix V Diamino/Carbon
clean-up mix
900 mg MgSO4
150 mg Diamino
45 mg Carbon
730975 50
    2 mL* Mix V Diamino/Carbon
clean-up mix
150 mg MgSO4
25 mg Diamino
7.5 mg Carbon
730358.2 50
 
15 mL* Mix VI Diamino/C18 ec
clean-up mix
900 mg MgSO4
150 mg Diamino
150 mg C18 ec
730974 50
    2 mL* Mix VI Diamino/C18 ec
clean-up mix
150 mg MgSO4
25 mg Diamino
25 mg C18 ec
730858.2 50
 
CHROMABOND® QuEChERS clean-up mixes (AOAC 2007.01)
    15 mL* Mix XX Diamino clean-up mix 1200 mg MgSO4
400 mg Diamino
730658 50
    2 mL* Mix XX Diamino clean-up mix 150 mg MgSO4
50 mg Diamino
730670.2 50
    15 mL* Mix XVII Diamino/Carbon
clean-up mix
1200 mg MgSO4
400 mg Diamino
400 mg Carbon
730842 50
 
2 mL* Mix XVII Diamino/Carbon
clean-up mix
150 mg MgSO4
50 mg Diamino
50 mg Carbon
730996.2 50
    15 mL* Mix XIX Diamino/C18 ec
clean-up mix
1200 mg MgSO4
400 mg Diamino
400 mg C18 ec
730669 50
 
15 mL* Mix XIX Diamino/C18 ec
clean-up mix
150 mg MgSO4
50 mg Diamino
50 mg C18 ec
730657 50
    2 mL* Mix XIX Diamino/C18 ec
clean-up mix
150 mg MgSO4
50 mg Diamino
50 mg C18 ec
730657.2 50
    15 mL* Mix XLVII
Diamino/Carbon/C18 ec
clean-up mix
1200 mg MgSO4
400 mg Diamino
400 mg Carbon
400 mg C18 ec
730845 50
    2 mL* Mix XLVII
Diamino/Carbon/C18 ec
clean-up mix
150 mg MgSO4
50 mg Diamino
50 mg Carbon
50 mg C18 ec
730843.2 50
  CHROMABOND® Diamino polypropylene columns
  3 mL   adsorbent weight 200 mg   730561 50
  6 mL   adsorbent weight 500 mg   730562 30
  CHROMABOND® Diamino adsorbent
          730653.20 20 g
          730653 100 g
    CHROMABOND® QuEChERS accessories
    50 mL polypropylene centrifuge tube with screw cap 730223 50
* 2 mL centrifuge tube with snap cap or 15 mL centrifuge tube with screw cap
(50 mL centrifuge tubes as well as aluminium bags on request)
A number of custom-made QuEChERS mixes is available on request.

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