|
|
|
|
| NucleoMag® 96 Tissue |
Genomic DNA from tissue and cells
|
|
| Features |
|
| Magnetic-bead based isolation of genomic DNA from human or animal tissue, cultured cells, or bacteria |
|
• One-tube procedure minimizes risk of cross-contamination
• Small elution volumes ≥ 50 μL possible
• Yield does not depend on elution volume
• Easily adapted to automated use
• Processing at room temperature (excluding lysis step)
|
| Technology |
Magnetic-bead technology |
| Format |
Highly reactive superparamagnetic beads |
| Processing |
Manual or automated
|
| Sample material |
< 20 mg tissue, < 107 cells or bacteria
|
| Fragment size |
300 bp–approx. 50 kbp |
| Typical yield |
10–20 µg |
| A260/A280 |
1.6–1.9 |
| Elution volume |
≥ 50 µL |
| Preparation time |
120 min/96 preps |
| Binding capacity |
Approx. 0.4 µg/µL beads |
|
|
Applications*
|
|
|
• Manual or automated isolation of DNA from human and animal tissue, cultured cells, or bacteria
• Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction
|
|
| * Kits to be used for research purposes only! |
|
|
| |
|
|
Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoMag® 96 Tissue |
1 x 96
|
NucleoMag® B-Beads, buffers, Proteinase K |
744300.1 |
|
4 x 96
|
as above |
744300.4 |
|
24 x 96
|
as above |
744300.24 |
|
|
|
|
| Material to be supplied by the user |
| Lysis tubes, e.g. Rack of Tube Strips |
4 sets
|
incl. Cap Strips |
740477 |
| Separation plate, e.g. Square-well Block |
4
|
|
740481 |
|
24
|
|
740481.24 |
| Elution plate, e.g. Elution Plate U-bottom |
24
|
|
740486.24 |
|
|
|
|
| For use with KingFisher® 96 platform |
|
|
|
| KingFisher® 96 Accessory Kit A |
1 set
|
Square-well Blocks, Deep-well Tip Combs and Elution Plates for 4 x 96 preparations |
744950 |
|
|
|
|
| Product accessories |
Pack of
|
Specification |
REF |
| NucleoMag® SEP |
1
|
magnetic separator |
744900 |
For ordering click here
|
| |
|
|
 |