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Instruction leaflet:
NUCLEOSIL®
RP columns


  
   
NUCLEOSIL® C4
Butyl modified silica phases for HPLC
Medium density modification, endcapping · ~ 2 % C · USP L26
Features
Endcapped phases for RP and ion-pairing chromatography
Available pore sizes 120, 300, 500, 1000 and 4000 Å, available particle sizes 5 µm, 7 µm and 10 µm
Separation mechanism based on hydrophobic (van der Waals) interactions, some residual silanol interactions
Retention times are shorter than on C8 and C18 phases
pH stability at 20 °C: 2–8
For applications click here.


EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length ->   125 mm 150 mm 250 mm   EC guard columns* CC guard columns**
NUCLEOSIL® 120-5 C4 · particle size 5 µm, pore size 120 Å, ~ 4 % C    
4.6 mm ID       720096.46   721083.30 721889.40
NUCLEOSIL® 300-5 C4 · particle size 5 µm, pore size 300 Å, ~ 2 % C    
4 mm ID   720901.40   720059.40   721916.30 721607.40
4.6 mm ID     720220.46 720059.46   721916.30 721607.40
Columns/pack   1 1 1   3 3
* EC 4/3 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with guard column adapter EC (REF 721359).
Custom-packed EC columns with other dimensions are available on request.


NUCLEOSIL® C4 bulk packings

Ordering information
Phase Carbon content Pore size Particle size Pack of 10 g Pack of 100 g
NUCLEOSIL 120-5 C4 ~ 4 % 120 Å 5 µm 712290.10 712290.100
NUCLEOSIL 300-5 C4 ~ 2 % 300 Å 5 µm 712620.10 712620.100
NUCLEOSIL 300-7 C4 ~ 2 % 300 Å 7 µm 712630.10 712630.100
NUCLEOSIL 300-10 C4 ~ 2 % 300 Å 10 µm 712640.10 712640.100
NUCLEOSIL 500-7 C4 < 1 % 500 Å 7 µm 712750.10 712750.100
NUCLEOSIL 1000-7 C4 < 1 % 1000 Å 7 µm 712780.10 712780.100
NUCLEOSIL 4000-7 C4 < 1 % 4000 Å 7 µm 712925.10 712925.100
 

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