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| NUCLEOSIL® SiOH |
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| Unmodified silica phases for HPLC · USP L3 |
| Features |
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Spherical silica, pH stability at 20 °C: 2–8
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
Separation mechanism based on polar interactions |
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Physical properties of unmodified NUCLEOSIL® SiOH materials |
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Phase |
Pore size |
Pore volume |
Surface (BET) |
Density |
Pressure stability* |
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NUCLEOSIL 50 |
50 Å |
0.8 mL/g |
420 m2/g |
0.45 g/mL |
600 bar |
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NUCLEOSIL 100 |
100 Å |
1 mL/g |
350 m2/g |
0.36 g/mL |
600 bar |
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NUCLEOSIL 120 |
120 Å |
0.65 mL/g |
200 m2/g |
0.55 g/mL |
500 bar |
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NUCLEOSIL 300 |
300 Å |
0.8 mL/g |
100 m2/g |
0.45 g/mL |
400 bar |
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NUCLEOSIL 500 |
500 Å |
0.8 mL/g |
35 m2/g |
0.45 g/mL |
400 bar |
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NUCLEOSIL 1000 |
1000 Å |
0.8 mL/g |
25 m2/g |
0.45 g/mL |
300 bar |
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NUCLEOSIL 4000 |
4000 Å |
0.7 mL/g |
10 m2/g |
0.48 g/mL |
300 bar |
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* Maximum packing pressure; the maximum working pressure for the EC columns listed below is 400 bar. |
| EC standard columns for analytical HPLC |
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| Ordering information |
| Eluent in column is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the columns with THF first. |
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Length -> |
250 mm |
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EC guard columns* |
CC guard columns** |
| NUCLEOSIL® 50-5 · particle size 5 µm, pore size 50 Å |
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| 4.6 mm ID |
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720093.46 |
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721167.30 |
721600.40 |
| NUCLEOSIL® 100-5 · particle size 5 µm, pore size 100 Å |
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| 4.6 mm ID |
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720099.46 |
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721518.30 |
721872.40 |
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Columns / pack |
1 |
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3 |
3 |
* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359). |
| Custom-packed EC columns with other dimensions are available on request. |
Unmodified NUCLEOSIL® SiOH bulk packings
Ordering information
| Phase |
Pore size |
Particle size |
Pack of 10 g |
Pack of 100 g |
| NUCLEOSIL 50-3 |
50 Å |
3 µm |
712000.10 |
712000.100 |
| NUCLEOSIL 50-5 |
50 Å |
5 µm |
712010.10 |
712010.100 |
| NUCLEOSIL 50-7 |
50 Å |
7 µm |
712020.10 |
712020.100 |
| NUCLEOSIL 50-10 |
50 Å |
10 µm |
712030.10 |
712030.100 |
| NUCLEOSIL 100-3 |
100 Å |
3 µm |
712360.10 |
712360.100 |
| NUCLEOSIL 100-5 |
100 Å |
5 µm |
712040.10 |
712040.100 |
| NUCLEOSIL 100-7 |
100 Å |
7 µm |
712050.10 |
712050.100 |
| NUCLEOSIL 100-10 |
100 Å |
10 µm |
712060.10 |
712060.100 |
| NUCLEOSIL 120-3 |
120 Å |
3 µm |
712390.10 |
719390.100 |
| NUCLEOSIL 120-5 |
120 Å |
5 µm |
712400.10 |
712400.100 |
| NUCLEOSIL 120-7 |
120 Å |
7 µm |
712410.10 |
712410.100 |
| NUCLEOSIL 120-10 |
120 Å |
10 µm |
712420.10 |
712420.100 |
| NUCLEOSIL 300-5 |
300 Å |
5 µm |
712430.10 |
712430.100 |
| NUCLEOSIL 300-7 |
300 Å |
7 µm |
712440.10 |
712440.100 |
| NUCLEOSIL 300-10 |
300 Å |
10 µm |
712450.10 |
712450.100 |
| NUCLEOSIL 500-5 |
500 Å |
5 µm |
712680.10 |
712680.100 |
| NUCLEOSIL 500-7 |
500 Å |
7 µm |
712690.10 |
712690.100 |
| NUCLEOSIL 500-10 |
500 Å |
10 µm |
712700.10 |
712700.100 |
| NUCLEOSIL 1000-5 |
1000 Å |
5 µm |
712710.10 |
712710.100 |
| NUCLEOSIL 1000-7 |
1000 Å |
7 µm |
712720.10 |
712720.100 |
| NUCLEOSIL 1000-10 |
1000 Å |
10 µm |
712730.10 |
712730.100 |
| NUCLEOSIL 4000-5 |
4000 Å |
5 µm |
712850.10 |
712850.100 |
| NUCLEOSIL 4000-7 |
4000 Å |
7 µm |
712860.10 |
712860.100 |
| NUCLEOSIL 4000-10 |
4000 Å |
10 µm |
712870.10 |
712870.100 |
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