NUCLEODUR C18 Isis

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Instruction leaflet:
NUCLEODUR®
RP columns

NUCLEODUR® C18 Isis
High purity silica phase for HPLC
Nonpolar crosslinked polymeric octadecyl modification high steric selectivity · endcapped · 20 % C · USP L1
Properties

	Key features: Exceptional steric selectivity Outstanding surface deactivation Suitable for LC/MS and HPLC at pH extremes (pH 1–10)
	Technical characteristics: C18 phase with special polymeric, crosslinked surface modification pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm; carbon content 20 %
	Recommended application: Steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins For applications click here.

Standard program* of columns packed with NUCLEODUR® C18 Isis
Column system		Particle size:	1.8 µm	3 µm	5 µm
Analytical	EC columns (standard system, 2–4.6 mm)
	Microbore columns (MB, 1 mm)
Preparative	VarioPrep columns (VP, ≥ 8 mm)
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water

Length ->	30 mm	50 mm	75 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR® C18 Isis, 1.8 µm
2 mm ID	760406.20	760405.20	760396.20	760407.20		760409.20
3 mm ID	760406.30	760405.30		760407.30
4 mm ID	760406.40	760405.40		760407.40
4.6 mm ID	760406.46	760405.46		760407.46
EC guard columns*:		4 x 2 mm	761910.20		4 x 3 mm	761910.30
NUCLEODUR® C18 Isis, 3 µm
2 mm ID		760400.20		760401.20	760402.20	760403.20	760404.20
3 mm ID		760400.30		760401.30	760402.30	760403.30	760404.30
4 mm ID		760400.40		760401.40	760402.40	760403.40	760404.40
4.6 mm ID		760400.46	760397.46	760401.46	760402.46	760403.46	760404.46
EC guard columns*:		4 x 2 mm	761911.20		4 x 3 mm	761911.30
CC guard columns**:		8 x 3 mm	761300.30		8 x 4 mm	761300.40
NUCLEODUR® C18 Isis, 5 µm
2 mm ID		760410.20		760415.20	760412.20	760413.20	760414.20
3 mm ID		760410.30		760415.30	760412.30	760413.30	760414.30
4 mm ID		760410.40		760415.40	760412.40	760413.40	760414.40
4.6 mm ID		760410.46	760416.46	760415.46	760412.46	760413.46	760414.46
EC guard columns*:		4 x 2 mm	761912.20		4 x 3 mm	761912.30
CC guard columns**:		8 x 3 mm	761310.30		8 x 4 mm	761310.40
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns			EC columns with ID				Use guard column holder
			2 mm	3 mm	4 mm	4.6 mm	Use guard column holder
*	Column Protection System	EC guard column	4/2	4/3	4/3	4/3	REF 718966
**	ChromCart® guard column system	CC guard column	8/3	8/3	8/4	8/4	REF 721359
Custom-packed EC columns with other dimensions are available on request.
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Microbore columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water

Length ->	50 mm	100 mm	125 mm
NUCLEODUR® C18 Isis, 3 µm
1 mm ID	717760.10	717761.10	717762.10
NUCLEODUR® C18 Isis, 5 µm
1 mm ID	717770.10	717771.10	717772.10
All columns in packs of 1. Guard columns for microbore columns on request!
On request, Microbore columns are also available in lengths of 40, 60, 200, 250, and 300 mm and with 0.05 · 0.075 · 0.1 · 0.15 · 0.3 · 0.4 · 0.5 · 0.75 and 1.5 mm ID.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water

Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR® C18 Isis, 5 µm
50 mm	762404.100	762404.210
125 mm	762405.100	762405.210
150 mm				762406.400
250 mm	762403.100	762403.210	762403.320	762403.400
VP guard columns with NUCLEODUR® C18 Isis
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762420.80	762420.160
15 mm*			762422.320

* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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