NUCLEODUR Sphinx RP

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Instruction leaflet:
NUCLEODUR®
RP columns

NUCLEODUR® Sphinx RP
High purity silica phase for HPLC
Bifunctional RP phase, balanced ratio of propylphenyl and C18 ligands endcapped · 15 % C · USP L1 / L11
Properties

	Key features: Distinct selectivity based on well-balanced bifunctional surface coverage Widens the scope for method development based on additional π-π interactions Suitable for LC/MS due to low bleeding characteristics
	Technical characteristics: Octadecyl and propylphenyl modified silica pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm carbon content 15 %; pH stability 1–10; high reproducibility
	Recommended application: Quinolone antibiotics, sulfonamides, xanthines, substituted aromatics For applications click here.

Standard program* of columns packed with NUCLEODUR® Sphinx RP
Column system		Particle size:	1.8 µm	3 µm	5 µm
Analytical	EC columns (standard system, 2–4.6 mm)
Preparative	VarioPrep columns (VP, ≥ 8 mm)
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water

Length ->	30 mm	50 mm	75 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR® Sphinx RP, 1.8 µm
2 mm ID	760821.20	760822.20	760825.20	760823.20		760824.20
3 mm ID	760821.30	760822.30		760823.30
4 mm ID	760821.40	760822.40		760823.40
4.6 mm ID	760821.46	760822.46		760823.46
EC guard columns*:		4 x 2 mm	761920.20		4 x 3 mm	761920.30
NUCLEODUR® Sphinx RP, 3 µm
2 mm ID		760806.20		760812.20	760807.20	760805.20	760808.20
3 mm ID		760806.30		760812.30	760807.30	760805.30	760808.30
4 mm ID		760806.40		760812.40	760807.40	760805.40	760808.40
4.6 mm ID		760806.46	760813.46	760812.46	760807.46	760805.46	760808.46
EC guard columns*:		4 x 2 mm	761921.20		4 x 3 mm	761921.30
CC guard columns**:		8 x 3 mm	761557.30		8 x 4 mm	761557.40
NUCLEODUR® Sphinx RP, 5 µm
2 mm ID		760800.20		760809.20	760801.20	760802.20	760803.20
3 mm ID		760800.30		760809.30	760801.30	760802.30	760803.30
4 mm ID		760800.40		760809.40	760801.40	760802.40	760803.40
4.6 mm ID		760800.46	760815.46	760809.46	760801.46	760802.46	760803.46
EC guard columns*:		4 x 2 mm	761922.20		4 x 3 mm	761922.30
CC guard columns**:		8 x 3 mm	761550.30		8 x 4 mm	761550.40
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns			EC columns with ID				Use guard column holder
			2 mm	3 mm	4 mm	4.6 mm	Use guard column holder
*	Column Protection System	EC guard column	4/2	4/3	4/3	4/3	REF 718966
**	ChromCart® guard column system	CC guard column	8/3	8/3	8/4	8/4	REF 721359
Custom-packed EC columns with other dimensions are available on request.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water

Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR® Sphinx RP, 5 µm
50 mm	762372.100	762372.210
125 mm	762375.100	762375.210
150 mm				762371.400
250 mm	762373.100	762373.210	762373.320	762373.400
VP guard columns with NUCLEODUR® Sphinx RP
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762390.80	762390.160
15 mm*			762392.320

* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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