NUCLEODUR C18 Isis

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Instruction leaflet:
NUCLEODUR®
RP columns

NUCLEODUR® C18 Isis
High purity silica phase for HPLC
Nonpolar crosslinked polymeric octadecyl modification high steric selectivity · endcapped · 20 % C · USP L1
Properties

	Key features: Exceptional steric selectivity Outstanding surface deactivation Suitable for LC/MS and HPLC at pH extremes (pH 1–10)
	Technical characteristics: C18 phase with special polymeric, crosslinked surface modification pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm; carbon content 20 %
	Recommended application: Steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins For applications click here.

Standard program* of columns packed with NUCLEODUR® C18 Isis
Column system		Particle size:	1.8 µm	3 µm	5 µm
Analytical	EC columns (standard system, 2–4.6 mm)
Preparative	VarioPrep columns (VP, ≥ 8 mm)
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water

Length ->	30 mm	50 mm	75 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR® C18 Isis, 1.8 µm
2 mm ID	760406.20	760405.20	760396.20	760407.20		760409.20
3 mm ID	760406.30	760405.30		760407.30
4 mm ID	760406.40	760405.40		760407.40
4.6 mm ID	760406.46	760405.46		760407.46
EC guard columns*:		4 x 2 mm	761910.20		4 x 3 mm	761910.30
NUCLEODUR® C18 Isis, 3 µm
2 mm ID		760400.20		760401.20	760402.20	760403.20	760404.20
3 mm ID		760400.30		760401.30	760402.30	760403.30	760404.30
4 mm ID		760400.40		760401.40	760402.40	760403.40	760404.40
4.6 mm ID		760400.46	760397.46	760401.46	760402.46	760403.46	760404.46
EC guard columns*:		4 x 2 mm	761911.20		4 x 3 mm	761911.30
CC guard columns**:		8 x 3 mm	761300.30		8 x 4 mm	761300.40
NUCLEODUR® C18 Isis, 5 µm
2 mm ID		760410.20		760415.20	760412.20	760413.20	760414.20
3 mm ID		760410.30		760415.30	760412.30	760413.30	760414.30
4 mm ID		760410.40		760415.40	760412.40	760413.40	760414.40
4.6 mm ID		760410.46	760416.46	760415.46	760412.46	760413.46	760414.46
EC guard columns*:		4 x 2 mm	761912.20		4 x 3 mm	761912.30
CC guard columns**:		8 x 3 mm	761310.30		8 x 4 mm	761310.40
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns			EC columns with ID				Use guard column holder
			2 mm	3 mm	4 mm	4.6 mm	Use guard column holder
*	Column Protection System	EC guard column	4/2	4/3	4/3	4/3	REF 718966
**	ChromCart® guard column system	CC guard column	8/3	8/3	8/4	8/4	REF 721359
Custom-packed EC columns with other dimensions are available on request.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water

Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR® C18 Isis, 5 µm
50 mm	762404.100	762404.210
125 mm	762405.100	762405.210
150 mm				762406.400
250 mm	762403.100	762403.210	762403.320	762403.400
VP guard columns with NUCLEODUR® C18 Isis
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762420.80	762420.160
15 mm*			762422.320

* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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