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NUCLEOSIL® 4000-7 PEI
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silica-based HPLC phase for anion exchange chromatography of proteins and peptides
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Features
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base material NUCLEOSIL® silica, particle size 7 µm, pore size 4000 Å
polymeric, covalently bonded polyethyleneimine network, weakly basic anion exchanger
ion exchange capacity 0.15 mmol/g; protein binding capacity 61 mg BSA/g
pH stability 2 – 8.5; max. working pressure 250 bar
separation principle: reversible adsorption of negatively charged substances to positively charged groups on the exchanger material and their subsequent displacement by either increasing ionic strength or pH changes in the mobile phase
eluent in column methanol
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high selectivity for numerous proteins: e. g β-lactoglobulins A and B,
two proteins differing in just two amino acids, can be separated in
only 10 minutes;
biological activity of purified proteins is preserved
good binding and desorption kinetics for nucleotides as well |
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Ordering information
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Length
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50 mm
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125 mm
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250 mm
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Guard columns*
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NUCLEOSIL® 4000-7 PEI
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| EC analytical columns |
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4 mm ID
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720401.40
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720402.40
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720403.40
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721091.40
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| VarioPrep preparative columns |
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10 mm ID
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715230.100
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715231.100
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* 8 x 4 mm ChromCart® guard column cartridges are coupled to EC columns using guard column adaptor EC
(REF 721359). |
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