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| NUCLEOSIL® PPN |
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silica-based HPLC phases for RP chromatography
of biological macromolecules
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Features
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silica-based reversed phase materials with polymerically bonded alkyl chains
exclusively hydrophobic interactions
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NUCLEOSIL® 100-5 C18 PPN: octadecyl phase, particle size 5 µm, pore size 100 Å ∙ USP L1
dynamic protein binding capacity per g packing: 8 mg BSA, 64 mg cytochrome C
suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides
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NUCLEOSIL® 500-5 C18 PPN: octadecyl phase, particle size 5 µm, pore size 500 Å ∙ USP L1
dynamic protein binding capacity per g packing: 22 mg BSA, 40 mg cytochrome C
especially suited for large peptides and medium-size hydrophilic proteins
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pH working range 1 – 9, max. working pressure 250 bar
eluent in column methanol
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Ordering information
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Length
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50 mm
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125 mm
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250 mm
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Guard columns*
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NUCLEOSIL® 100-5 C18 PPN
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| EC analytical columns |
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4 mm ID
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720250.40
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720251.40
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720252.40
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721594.40
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| VarioPrep preparative columns |
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10 mm ID
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715326.100
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715325.100
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| NUCLEOSIL® 500-5 C18 PPN |
| EC analytical columns |
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4 mm ID
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720256.40
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720257.40
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720258.40
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721687.40 |
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| VarioPep preparative columns |
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10 mm ID
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715318.100
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715316.100
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* 8 x 4 mm ChromCart guard column cartridges are coupled to EC columns using guard column adaptor EC
(Cat. No. 721359). |
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