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NUCLEOSIL® PPN
Silica-based HPLC phases for RP chromatography of biological macromolecules
Features
Silica-based reversed phase materials with polymerically bonded alkyl chains,
exclusively hydrophobic interactions
 
NUCLEOSIL® 100-5 C18 PPN: octadecyl phase, particle size 5 µm, pore size 100 Å ∙ USP L1
Dynamic protein binding capacity per g packing: 8 mg BSA, 64 mg cytochrome C
Suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides
NUCLEOSIL® 500-5 C18 PPN: octadecyl phase, particle size 5 µm, pore size 500 Å ∙ USP L1
Dynamic protein binding capacity per g packing: 22 mg BSA, 40 mg cytochrome C
Especially suited for large peptides and medium-size hydrophilic proteins
For more applications click here.
pH working range 1–9, max. working pressure 250 bar
Eluent in column methanol
 


Ordering information
Length -> 50 mm 125 mm 250 mm   CC guard columns*
EC analytical columns       
NUCLEOSIL® 100-5 C18 PPN    
4 mm ID 720250.40 720251.40 720252.40   721594.40
NUCLEOSIL® 500-5 C18 PPN    
4 mm ID 720256.40 720257.40 720258.40   721687.40
     
* CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359).
Guard columns in packs of 2, EC columns in packs of 1

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