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| NUCLEOSIL® MPN |
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Silica-based HPLC phases for RP chromatography
of biological macromolecules |
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Features
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Silica-based reversed phase materials with monomerically bonded alkyl chains, brush type structure
predominantly hydrophobic forces with a small portion of hydrophilic interactions |
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NUCLEOSIL® 100-5 C18 MPN: octadecyl phase, particle size 5 µm, pore size 100 Å ∙ USP L1
Dynamic protein binding capacity per g packing: 6 mg BSA, 110 mg cytochrome C |
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NUCLEOSIL® 120-3 C18 MPN: octadecyl phase, particle size 3 µm, pore size 120 Å ∙ USP L1
Dynamic protein binding capacity per g packing: 16 mg BSA, 55 mg cytochrome C
outstanding selectivity for peptides |
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NUCLEOSIL® 300-5 C4 MPN: butyl phase, particle size 5 µm, pore size 300 Å ∙ USP L26
Dynamic protein binding capacity per g packing: 14 mg BSA, 27 mg cytochrome C
especially suited for the purification of larger, hydrophobic peptides and very different proteins
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pH working range 2–8, max. working pressure 250 bar
Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.
Eluent in column methanol |
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Ordering information
| Length -> |
50 mm |
125 mm |
250 mm |
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CC guard columns* |
EC analytical columns  |
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| NUCLEOSIL® 100-5 C18 MPN |
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| 4 mm ID |
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720230.40 |
720231.40 |
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| NUCLEOSIL® 120-3 C18 MPN |
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| 4 mm ID |
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720232.40 |
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| NUCLEOSIL® 300-5 C4 MPN |
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| 4 mm ID |
720244.40 |
720045.40 |
720245.40 |
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721113.40 |
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| * CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359). Guard column cartridges in packs of 2, EC columns in packs of 1 |
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