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| NUCLEOSIL® MPN |
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silica-based HPLC phases for RP chromatography
of biological macromolecules
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Features
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silica-based reversed phase materials with monomerically bonded alkyl chains, brush type structure
predominantly hydrophobic forces with a small portion of hydrophilic interactions
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NUCLEOSIL® 100-5 C18 MPN: octadecyl phase, particle size 5 µm, pore size 100 Å ∙ USP L1
dynamic protein binding capacity per g packing: 6 mg BSA, 110 mg cytochrome C
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NUCLEOSIL® 120-3 C18 MPN: octadecyl phase, particle size 3 µm, pore size 120 Å ∙ USP L1
dynamic protein binding capacity per g packing: 16 mg BSA, 55 mg cytochrome C
outstanding selectivity for peptides
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NUCLEOSIL® 300-5 C4 MPN: butyl phase, particle size 5 µm, pore size 300 Å ∙ USP L26
dynamic protein binding capacity per g packing: 14 mg BSA, 27 mg cytochrome C
especially suited for the purification of larger, hydrophobic peptides and very different proteins
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pH working range 2 – 8, max. working pressure 250 bar
maximum
separation efficiency can be achieved when the injected protein mass
does not exceed 1 – 2 % of the maximum protein loading capacity
eluent in column methanol
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Ordering information
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Length
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50 mm
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125 mm
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250 mm
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Guard columns*
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NUCLEOSIL® 100-5 C18 MPN
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| EC analytical columns |
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4 mm ID
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720230.40
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720231.40
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| NUCLEOSIL® 120-3 C18 MPN |
| EC analytical columns |
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4 mm ID
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720232.40
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| NUCLEOSIL® 300-5 C4 MPN |
| EC analytical columns |
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4 mm ID
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720244.40
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720045.40
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720245.40
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721113.40 |
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* 8 x 4 mm ChromCart® guard column cartridges are coupled to EC columns using guard column adaptor EC
(REF 721359). |
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