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| NUCLEOGEN® DEAE |
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silica-based HPLC phases
for anion exchange chromatography of nucleic acids
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Features
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base material silica, particle size 7 µm
DEAE anion exchanger
eluent in column methanol
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NUCLEOGEN® 60-7 DEAE: pore size 60 Å
for separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95%
capacity 200 A260/ml (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column)
preparative separations possible when using higher flow rates and longer gradient times
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NUCLEOGEN® 500-7 DEAE: pore size 500 Å
for the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000 – 1,000,000 daltons) with recoveries > 95%
capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column
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NUCLEOGEN® 4000-7 DEAE: pore size 4000 Å
for the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e. very high molecular weight nucleic acids (e.g. 1 – 50 megadaltons)
capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column
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Ordering information
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Length
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125 mm
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Guard columns*
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| NUCLEOGEN® 60-7 DEAE |
| EC analytical columns |
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4 mm ID
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736596.40
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736400.40
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| VarioPrep preparative columns |
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10 mm ID
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736597.100
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736400.40 |
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| NUCLEOGEN® 500-7 DEAE |
| Valco type analytical columns |
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6 mm ID
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736598
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736400.40 |
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| VarioPep preparative columns |
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10 mm ID
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736599.100
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736400.40 |
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| NUCLEOGEN® 4000-7 DEAE |
| Valco type analytical columns |
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6 mm ID
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736601
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736400.40 |
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| VarioPrep preparative columns |
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10 mm ID
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736602.100
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736400.40 |
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| * ChromCart NUCLEOGEN guard column cartridges measure 30 x 4 mm and are supplied in packs of 2. They require the CC column holder 30 mm (REF 721823) |
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