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NUCLEOSIL® CHIRAL-1
NUCLEOSIL® CHIRAL-2
NUCLEOSIL® CHIRAL-3 |
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| Silica-based phases for HPLC enantiomer separation |
| Features |
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NUCLEOSIL® CHIRAL-1
for enantiomer separation based on ligand exchange · USP L32 Base material NUCLEOSIL ® silica, particle size 5 μm, pore size 120 Å,
chiral selector L-hydroxyproline – Cu 2+ complexes Principal interaction mode:
formation of ternary mixed-ligand complexes with Cu(II) ions
Differences in the stability of the diastereomeric complexes cause chromatographic separation. Eluent in column 0.5 mM copper sulfate solution Recommended application: enantiomers with two polar functional groups with the correct spacing such as α-amino acids, α-hydroxycarboxylic acids (e.g., lactic acid), N-alkyl-α-amino acids etc. |
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NUCLEOSIL® CHIRAL-2 / NUCLEOSIL® CHIRAL-3
for enantiomer separation in organic eluent systems · USP L36Base material NUCLEOSIL ® silica, particle size 5 μm, pore size 100 Å,
chiral selector for NUCLEOSIL ® CHIRAL-2: N-(3,5-dinitrobenzoyl)-D-phenylglycine, for NUCLEOSIL ® CHIRAL-3 the optical antipode of the CHIRAL-2 phase, "brush type" phases Principle interaction modes:
charge-transfer interactions, hydrogen bonds, dipole-dipole interactions and steric effects Eluent in column n-heptane – 2-propanol – TFAA (100:0.05:0.05) Recommended application: analysis of stereoisomers such as separation of
enantiomers and diastereomers, control of optical purity of plant
protectives (pesticides, e.g., propionic acid derived herbicides), pharmaceuticals etc. and for product control in chiral organic syntheses |
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For control of the optical purity of a substance, the two columns NUCLEOSIL® CHIRAL-2 and NUCLEOSIL®
CHIRAL-3 allow to select conditions such that the minor enantiomer,
which is present as an impurity, is eluted before the main peak. Thus,
overlapping peaks are avoided. This makes an exact quantification of the
impurity much easier. |
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For more applications click here. |
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Ordering information
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Length 250 mm |
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EC guard columns* |
CC guard column** |
EC analytical columns  |
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| NUCLEOSIL® CHIRAL-1 |
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| 4 mm ID |
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720081.40 (1/pack) |
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721188.30 (1/pack) |
721455.40 (1/pack) |
| NUCLEOSIL® CHIRAL-2 |
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| 4 mm ID |
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720088.40 (1/pack) |
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721190.30 (1/pack) |
721458.40 (3/pack) |
| NUCLEOSIL® CHIRAL-3 |
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| 4 mm ID |
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720350.40 (1/pack) |
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721190.30 (1/pack) |
721458.40 (3/pack) |
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* EC 4/3 guard column cartridges are used for EC columns of 4 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359).
Guard column cartridges for NUCLEOSIL® CHIRAL-2 and CHIRAL-3 are identical. |
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