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NUCLEOSIL® CHIRAL-1
NUCLEOSIL® CHIRAL-2
NUCLEOSIL® CHIRAL-3
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silica-based phases for HPLC enantiomer separation
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Features
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NUCLEOSIL® CHIRAL-1
for enantiomer separation based on ligand exchange · USP L32
base material NUCLEOSIL® silica, particle size 5 μm, pore size 120 Å
chiral selector L-hydroxyproline / Cu2+ complexes
principal interaction mode:
formation of ternary mixed-ligand complexes with Cu(II) ions
differences in the stability of the diastereomeric complexes cause chromatographic separation
eluent in column 0.5 mM copper sulphate solution
recommended application: enantiomers with two polar functional groups with the correct spacing such as α-amino acids, α-hydroxycarboxylic acids (e. g. lactic acid), N-alkyl-α-amino acids etc.
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NUCLEOSIL® CHIRAL-2 /
NUCLEOSIL® CHIRAL-3
for enantiomer separation in organic eluent systems · USP - / L36
base material NUCLEOSIL® silica, particle size 5 μm, pore size 100 Å
chiral selector for NUCLEOSIL® CHIRAL-2 is N-(3,5-dinitrobenzoyl)-D-phenylglycine, for NUCLEOSIL® CHIRAL-3 the optical antipode of the CHIRAL-2 phase,“brush type” phases
principle interaction modes:
charge-transfer interactions, hydrogen bonds, dipole-dipole interactions and steric effects
eluent in column n-heptane / 2-propanol / TFAA 100:0.5:0.5
recommended application: analysis of stereoisomers such as separation of enantiomers and diastereomers, control of optical purity of plant protectives (pesticides, e. g. propionic acid derived herbicides), pharmaceuticals etc. and for product control in chiral organic syntheses
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For control of the optical purity of a substance, the two columns NUCLEOSIL® CHIRAL-2 and NUCLEOSIL® CHIRAL-3 allow to select conditions such that the minor enantiomer, which is present as an impurity, is eluted before the main peak. Thus, overlapping peaks are avoided. This makes an exact quantification of the impurity much easier.
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Ordering information
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Length
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250 mm
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Guard columns
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| NUCLEOSIL® CHIRAL-1 |
| EC analytical columns |
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4 mm ID
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720081.40
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721455.40
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NUCLEOSIL® CHIRAL-2
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| EC analytical columns |
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4 mm ID
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720088.40
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721458.40* |
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| NUCLEOSIL® CHIRAL-3 |
| EC analytical columns |
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4 mm ID
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720350.40
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721458.40* |
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* 8 x 4 mm ID ChromCart® guard column cartridges for NUCLEOSIL® CHIRAL-2 and CHIRAL-3 are identical and supplied in packs of 3, the EC columns in packs of 1.
Guard column cartridges are coupled to EC columns using guard column adaptor EC (Cat. No. 721359).
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