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| NUCLEODEX |
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Cyclodextrin-modified silica phases
for HPLC enantiomer separation |
| Features |
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Base material NUCLEOSIL® silica, particle size 5 μm, pore size 100 Å
modified cyclodextrins as chiral selectors |
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NUCLEODEX β-OH: β-cyclodextrin (R = H; n = 2) ∙ USP L45
Separation based on hydrogen bonds and dipole interactions between
functional groups of the analyte and hydroxyl groups of the cyclodextrin
Examples for successful enantiomer separations: chlorthalidone and other compounds, which require free hydroxyl groups for enantioselective interactions
Eluent in column CH3OH – 0.1 % TEAA pH 4 (55:45) |
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NUCLEODEX α-PM: permethylated α-cyclodextrin (R = CH3; n = 1)
For all permethylated phases the ability to form hydrogen bonds is reduced, the hydrophobicity of the phase is increased compared to β‑OH, resulting in shorter retention times .
Examples for successful enantiomer separations: mecoprop and dichlorprop as free carboxylic acids, trans-stilbene oxide and styrene oxide
Eluent in column CH3OH – 50 mM phosphate pH 3 (70:30) |
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NUCLEODEX β-PM:
permethylated β-cyclodextrin (R = CH3; n = 2) ∙ USP L45
Examples for successful enantiomer separations:
mephobarbital (prominal), pesticides derivatives mecoprop-methyl et fenoxaprop-ethyl
Eluent in column CH3OH – 0.1 % TEAA pH 4 (65:35) |
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NUCLEODEX γ-PM:
permethylated γ-cyclodextrin (R = CH3; n = 3)
Examples for successful enantiomer separations:
steroids or other larger molecules
Eluent in column CH3OH – 0.1 % TEAA pH 4 (55:45) |
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NUCLEODEX phases are especially suited for the control of optical purity, but also for semipreparative separations and for the analysis of positional and cis-trans isomers.
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Ordering information
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Length 200 mm |
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EC guard columns* |
CC guard columns** |
EC analytical columns  |
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| NUCLEODEX β-OH |
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| 4 mm ID |
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720124.40 |
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721171.30 |
721460.40 |
| NUCLEODEX α-PM |
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| 4 mm ID |
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720127.40 |
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721469.30 |
721464.40 |
| NUCLEODEX β-PM |
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| 4 mm ID |
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720125.40 |
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721176.30 |
721462.40 |
| NUCLEODEX γ-PM |
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| 4 mm ID |
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720752.40 |
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721178.30 |
721466.40 |
| NUCLEODEX screening kit |
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consists of one CC 30/4 each with NUCLEODEX β-OH, α-PM, β-PM and γ-PM
and a CC column holder 30 mm |
721920 |
| All columns and guard column cartridges in packs of 1 |
* EC 4/3 guard column cartridges are used for EC columns of 4 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359).
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