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| NUCLEODEX |
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cyclodextrin-modified silica phases
for HPLC enantiomer separation
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Features
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base material NUCLEOSIL® silica, particle size 5 μm, pore size 100 Å
modified cyclodextrins as chiral selectors
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NUCLEODEX β-OH: β-cyclodextrin (R = H; n = 2) ∙ USP L45
separation based on hydrogen bonds and dipole interactions between functional groups of the analyte and hydroxyl groups of the cyclodextrin
examples for successful enantiomer separations: chlorthalidone and other compounds, which require free hydroxyl groups for enantioselective interactions
eluent in column CH3OH / 0.1 % TEAA pH 4 (55:45)
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NUCLEODEX α-PM: permethylated α-cyclodextrin (R = CH3; n = 1)
for all permethylated phases the ability to form hydrogen bonds is reduced, the hydrophobicity of the phase is increased compared to β‑OH, resulting in shorter retention times
examples for successful enantiomer separations: mecoprop and dichlorprop as free carboxylic acids, trans-stilbene oxide, styrene oxide
eluent in column CH3OH / 50 mM phosphate pH 3 (70:30)
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NUCLEODEX β-PM:
permethylated β-cyclodextrin (R = CH3; n = 2) ∙ USP L45
examples for successful enantiomer separations:
mephobarbital (prominal), pesticide derivatives mecoprop methyl and fenoxaprop ethyl
eluent in column CH3OH / 0.1 % TEAA pH 4 (65:35)
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NUCLEODEX γ-PM:
permethylated γ-cyclodextrin (R = CH3; n = 3)
examples for successful enantiomer separations:
steroids or other larger molecules
eluent in column CH3OH / 0.1% TEAA pH 4 (55:45)
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NUCLEODEX phases are especially suited for the control of optical purity, but also for semipreparative separations and for the analysis of positional and cis-trans isomers.
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Ordering information
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Length
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200 mm
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Guard columns*
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| NUCLEODEX β-OH |
| EC analytical columns |
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4 mm ID
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720124.40
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721460.40
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NUCLEODEX α-PM
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| EC analytical columns |
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4 mm ID
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720127.40
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721464.40 |
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| NUCLEODEX β-PM |
| EC analytical columns |
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4 mm ID
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720125.40
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721462.40 |
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NUCLEODEX γ-PM
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| EC analytical columns |
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4 mm ID
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720752.40
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721466.40 |
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| NUCLEODEX screening kit |
consists of one CC 30/4 each with NUCLEODEX β-OH, α-PM, β-PM and γ-PM
and a CC column holder 30 mm
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721920 |
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* 8 x 4 mm ChromCart guard column cartridges are coupled to EC columns using guard column adaptor EC
(REF 721359). |
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