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| NUCLEOSIL® standard C8 phases |
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| Octyl modified silica phases for HPLC |
C8 ec: medium density modification, endcapped · 9 % C · USP L7
C8: not encapped · USP L7 |
Features
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Nonpolar phases for RP chromatography
 NUCLEOSIL® C8 ec:
Endcapped, available pore sizes 50 and 100 Å, particle size 5 µm
Separation mechanism based on hydrophobic (van der Waals) interactions, slight residual silanol interactions
Corresponding NUCLEODUR ® phase: see NUCLEODUR® C8 ecNUCLEOSIL® C8:
Not endcapped, available pore sizes 100, 120, 300 and 500 Å,
available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
Separation mechanism based on hydrophobic (van der Waals) interactions, noticeable silanol interactions
pH stability at 20 °C for both types: 2–8; carbon content depends on phase (see tables) |
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For applications click here. |
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Standard NUCLEOSIL® C8 phases are available as:
Packed EC columns for analytical HPLC
Bulk packings |
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| EC standard columns for analytical HPLC |
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| Ordering information |
| Eluent in column acetonitrile – water |
| Length |
125 mm |
150 mm |
250 mm |
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EC guard columns* |
CC guard columns** |
| NUCLEOSIL® 50-5 C8 ec · 5 µm particles · 50 Å pores · 9 % C · endcapped |
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| 4.6 mm ID |
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720092.46 |
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721097.30 |
721834.40 |
| NUCLEOSIL® 100-5 C8 ec · 5 µm particles · 100 Å pores · 9 % C · endcapped |
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| 4 mm ID |
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720165.40 |
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721096.30 |
721805.40 |
| 4.6 mm ID |
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720165.46 |
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721096.30 |
721805.40 |
| NUCLEOSIL® 100-5 C8 · 5 µm particles · 100 Å pores · 8,5 % C · not endcapped |
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| 4 mm ID |
720001.40 |
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720013.40 |
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721194.30 |
721601.40 |
| 4.6 mm ID |
720001.46 |
720990.46 |
720013.46 |
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721194.30 |
721601.40 |
| NUCLEOSIL® 100-7 C8 · 7 µm particles · 100 Å pores · 8,5 % C · not endcapped |
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| 4 mm ID |
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720017.40 |
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| 4.6 mm ID |
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720017.46 |
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| NUCLEOSIL® 100-10 C8 · 10 µm particles · 100 Å pores · 8,5 % C · not endcapped |
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| 4 mm ID |
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720022.40 |
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| 4.6 mm ID |
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720022.46 |
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| NUCLEOSIL® 120-3 C8 · 3 µm particles · 120 Å pores · 6,5 % C · not endcapped |
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| 4 mm ID |
720071.40 |
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721093.30 |
721785.40 |
| 4.6 mm ID |
720071.46 |
720214.46 |
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721093.30 |
721785.40 |
| NUCLEOSIL® 120-5 C8 · 5 µm particles · 120 Å pores · 6,5 % C · not endcapped |
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| 4 mm ID |
720050.40 |
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720052.40 |
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721095.30 |
721787.40 |
| 4.6 mm ID |
720050.46 |
720735.46 |
720052.46 |
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721095.30 |
721787.40 |
| NUCLEOSIL® 300-5 C8 · 5 µm particles · 300 Å pores · ~ 3 % C · not endcapped |
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| 4.6 mm ID |
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720062.46 |
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721061.30 |
721101.40 |
| Columns/pack |
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1 |
1 |
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3 |
3 |
* EC 4/3 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with guard column adapter EC (REF 721359). |
| Custom-packed EC columns with other dimensions are available on request. |
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NUCLEOSIL® C8 bulk packings
Ordering information
| Phase |
Endcapped |
Carbon content |
Pore size |
Particle size |
Pack of 10 g |
Pack of 100 g |
| NUCLEOSIL 50-5 C8 ec |
yes |
9 % |
50 Å |
5 µm |
712032.10 |
712032.100 |
| NUCLEOSIL 100-5 C8 ec |
yes |
9 % |
100 Å |
5 µm |
712101.10 |
712101.100 |
| NUCLEOSIL 100-5 C8 |
no |
8.5 % |
100 Å |
5 µm |
712100.10 |
712100.100 |
| NUCLEOSIL 100-7 C8 |
no |
8.5 % |
100 Å |
7 µm |
712110.10 |
712110.100 |
| NUCLEOSIL 100-10 C8 |
no |
8.5 % |
100 Å |
10 µm |
712120.10 |
712120.100 |
| NUCLEOSIL 120-3 C8 |
no |
6.5 % |
120 Å |
3 µm |
712570.10 |
712570.100 |
| NUCLEOSIL 120-5 C8 |
no |
6.5 % |
120 Å |
5 µm |
712580.10 |
712580.100 |
| NUCLEOSIL 120-7 C8 |
no |
6.5 % |
120 Å |
7 µm |
712500.10 |
712500.100 |
| NUCLEOSIL 120-10 C8 |
no |
6.5 % |
120 Å |
10 µm |
712590.10 |
712590.100 |
| NUCLEOSIL 300-5 C8 |
no |
~ 3 % |
300 Å |
5 µm |
712650.10 |
712650.100 |
| NUCLEOSIL 300-7 C8 |
no |
~ 3 % |
300 Å |
7 µm |
712550.10 |
712550.100 |
| NUCLEOSIL 300-10 C8 |
no |
~ 3 % |
300 Å |
10 µm |
712660.10 |
712660.100 |
| NUCLEOSIL 500-7 C8 |
no |
< 1 % |
500 Å |
7 µm |
712830.10 |
712830.100 |
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