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Instruction leaflet:
NUCLEOSIL®
RP columns


NUCLEOSIL® standard C18 phases
Octadecyl modified silica phases for HPLC
endcapped · USP L1
Features
Nonpolar, endcapped phases for RP chromatography
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
pH stability at 20 °C: 2–8, carbon content depending on pore size (see table below)
Separation mechanism based on hydrophobic (van der Waals) interactions, slight residual silanol interactions
Corresponding NUCLEODUR® phases: see NUCLEODUR® C18 ec
For numerous applications click here.
Standard NUCLEOSIL® C18 phases are available as:
Packed EC columns for analytical HPLC
Packed VP columns for preparative HPLC

EC standard columns for HPLC
Ordering information
Eluent in column acetonitrile – water
ID Length   EC guard
columns*
CC guard
columns**
100 mm 125 mm 150 mm 250 mm
NUCLEOSIL® 50-5 C18 ec · 5 µm particles · 50 Å pores · 14.5 % C · endcapped    
4.6 mm       720098.46   721473.30 721829.40
NUCLEOSIL® 100-3 C18 · 3 µm particles · 100 Å pores · 15 % C · endcapped    
4 mm   720150.40   720133.40   721022.30 721866.40
4.6 mm 720841.46 720150.46 720949.46 720133.46   721022.30 721866.40
NUCLEOSIL® 100-5 C18 · 5 µm particles · 100 Å pores · 15 % C · endcapped    
2 mm   720002.20 720120.20 720014.20   721074.20 721602.30
3 mm   720002.30 720120.30 720014.30   721074.30 721602.30
4 mm 720141.40 720002.40 720120.40 720014.40   721074.30 721602.40
4.6 mm 720141.46 720002.46 720120.46 720014.46   721074.30 721602.40
NUCLEOSIL® 100-7 C18 · 7 µm particles · 100 Å pores · 15 % C · endcapped    
4 mm       720018.40      
4.6 mm   720951.46 720110.46 720018.46      
NUCLEOSIL® 100-10 C18 · 10 µm particles · 100 Å pores · 15 % C · endcapped    
4 mm       720023.40      
4.6 mm   720701.46 720140.46 720023.46      
NUCLEOSIL® 120-3 C18 · 3 µm particles · 120 Å pores · 11 % C · endcapped    
4 mm 720149.40 720040.40   720055.40   721075.30 721606.40
4.6 mm 720149.46 720040.46 720740.46 720055.46   721075.30 721606.40
NUCLEOSIL® 120-5 C18 · 5 µm particles · 120 Å pores · 11 % C · endcapped    
4 mm   720051.40   720041.40   721070.30 721783.40
4.6 mm   720051.46 720730.46 720041.46   721070.30 721783.40
NUCLEOSIL® 120-7 C18 · 7 µm particles · 120 Å pores · 11 % C · endcapped    
4 mm       720042.40      
NUCLEOSIL® 120-10 C18 · 10 µm particles · 120 Å pores · 11 % C · endcapped    
4 mm       720043.40      
4.6 mm       720043.46      
NUCLEOSIL® 300-5 C18 · 5 µm particles · 300 Å pores · 6.5 % C · endcapped    
4 mm       720065.40   721085.30 721608.40
4.6 mm       720065.46   721085.30 721608.40
NUCLEOSIL® 500-7 C18 · 7 µm particles · 500 Å pores · 2 % C · endcapped    
4.6 mm       720074.46      
NUCLEOSIL® 1000-7 C18 · 7 µm particles · 1000 Å pores · ~ 1 % C · endcapped    
4.6 mm       720077.46      
NUCLEOSIL® 4000-7 C18 · 7 µm particles · 4000 Å pores · < 1 % C · endcapped    
4.6 mm       720085.46      
Columns/pack -> 1 1 1 1   3 3
For EC columns with 2 mm ID 3 mm ID 4 mm ID 4.6 mm ID   REF
* EC guard columns EC 4/2 EC 4/3 EC 4/3 EC 4/3 use the Column Protection
System guard column holder
718966
** CC guard columns CC 8/3 CC 8/3 CC 8/4 CC 8/4 use the guard column
adapter EC
721359
Custom-packed EC columns with other dimensions are available on request.

VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water
Length 8 mm ID 10 mm ID 16 mm ID 21 mm ID    
NUCLEOSIL® 100-5 C18 · particle size 5 µm, pore size 100 Å · 15 % C · endcapped
250 mm   715340.100   715340.210    
NUCLEOSIL® 100-7 C18 · particle size 7 µm, pore size 100 Å · 15 % C · endcapped
250 mm 715332.80 715332.100 715332.160 715332.210    
NUCLEOSIL® 300-7 C18 · particle size 7 µm, pore size 300 Å · 6.5 % C · endcapped
250 mm   715806.100   715806.210    
Guard columns with NUCLEOSIL® C18
  8 mm ID 16 mm ID    
10 mm * 715360.80 715360.160    
* 10 x 8 mm ID guard column cartridges can be used for 8 and 10 mm ID VarioPrep columns and require the guard column holder 8 mm (REF 718251), 10 x 16 ID guard column cartridges can be used for 16 and 21 mm ID VarioPrep columns and require the guard column holder 16 mm (REF 718256)
Custom-packed VP columns with other dimensions are available on request.
Other NUCLEOSIL® octadecyl phases than those listed above can also be custom-packed in VarioPrep columns.

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