NUCLEOSIL CN

NUCLEOSIL® CN / CN-RP
Cyano (nitrile) modified silica phases for HPLC · USP L10
Features

Polar to mid-polar cyano (nitrile) modified silica for reversed phase and normal phase chromatography:
Normal phase: with low-polarity solvents for many compounds, which can also be separated on unmodified silica, however, due to the rapid equilibration much more suitable for gradient separations
Reversed phase: with different selectivity than C18, C8 or phenyl modified packings

Available pore sizes 100, 120, 300 and 500 Å, available particle sizes 5 µm, 7 µm and 10 µm,
pH stability at 20 °C: 2–8; carbon content 5 % for 100 Å pores, ~ 3 % for 120 Å pores

Separation mechanism based on π–π interactions, polar interactions and hydrophobic interactions

Corresponding NUCLEODUR® phase see NUCLEODUR® CN / CN-RP

For applications on NUCLEOSIL® CN phases click here:

EC standard columns for analytical HPLC
Ordering information
Eluent in column (except with NUCLEOSIL® 100-5 CN-RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.

ID	Particle size	Pore size	Length 250 mm	EC guard columns*	CC guard columns**
NUCLEOSIL® 100-5 CN (eluent in column n-heptane)
4 mm	5 µm	100 Å	720090.40	721078.30	721604.40
4.6 mm	5 µm	100 Å	720090.46	721078.30	721604.40
NUCLEOSIL® 100-5 CN-RP (eluent in column acetonitrile – water)
4 mm	5 µm	100 Å	720205.40	721039.30	721917.40
4.6 mm	5 µm	100 Å	720205.46	721039.30	721917.40
NUCLEOSIL® 100-10 CN (eluent in column n-heptane)
4 mm	10 µm	100 Å	720024.40
4.6 mm	10 µm	100 Å	720024.46
NUCLEOSIL® 120-7 CN (eluent in column n-heptane)
4 mm	7 µm	120 Å	720057.40
4.6 mm	7 µm	120 Å	720057.46
Columns/pack->			1	3	3
* EC 4/3 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with the Column Protection System guard column holder (REF 718966). ** CC 8/4 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with guard column adapter EC (REF 721359).
Custom-packed EC columns with other dimensions are available on request.

NUCLEOSIL® CN bulk packings

Ordering information
Phase	Carbon content	Pore size	Particle Size	Pack of 10 g	Pack of 100 g
NUCLEOSIL 100-5 CN	5 %	100 Å	5 µm	712160.10	712160.100
NUCLEOSIL 100-10 CN	5 %	100 Å	10 µm	712170.10	712170.100
NUCLEOSIL 120-7 CN	~ 3 %	120 Å	7 µm	712600.10	712600.100
NUCLEOSIL 300-7 CN	~ 2.5 %	300 Å	7 µm	712820.10	712820.100
NUCLEOSIL 500-7 CN	~ 2 %	500 Å	7 µm	712840.10	712840.100