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| NUCLEOSIL® NH2 / NH2 RP |
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| amino modified silica phases for HPLC · USP L8 |
| Features |
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polar aminopropyl modified silica for reversed phase and normal phase chromatography:
normal phase chromatography with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
reversed phase chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
anion exchange chromatography of anions and organic acids using common buffers (e.g. acetate or phosphate) in conjunction with organic modifiers (e.g. acetonitrile)
available pore sizes 100, 120 and 300 Å, available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions
pH stability at 20 °C: 2 – 8
Please note! Eluent in column is n-heptane. When using an eluent which is not miscible with n-heptane (e.g. water), it is necessary to rinse the column with THF first (exception: NUCLEOSIL® 100-5 NH2 RP). |
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for numerous applications on NUCLEOSIL® NH2 phases klick here:  |
| EC standard columns for analytical HPLC |
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| Ordering information |
| ID |
Particle size |
Pore size |
Length -> |
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250 mm |
Guard columns* |
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| NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile / water) |
| 4.6 mm |
5 µm |
100 Å |
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720095.46RP |
721605.40RP |
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| NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane) |
| 4.6 mm |
5 µm |
100 Å |
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720095.46 |
721605.40 |
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| NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane ) |
| 4.6 mm |
10 µm |
100 Å |
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720025.46 |
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Columns/pack |
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1 |
3 |
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* Guard column cartridges are coupled to EC columns using guard column adaptor EC (REF 721359).
4 mm ID (.40) CC guard column cartridges are applied for 4.6 mm ID EC columns. |
| Custom-packed EC columns with other dimensions are available on request. |
NUCLEOSIL® NH2 bulk packings
Ordering information
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Carbon content |
Pore size |
Particle Size |
Pack of 10 g |
Pack of 100 g |
| NUCLEOSIL 100-5 NH2 |
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3.5 % |
100 Å |
5 µm |
712200.10 |
712200.100 |
| NUCLEOSIL 100-10 NH2 |
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3.5 % |
100 Å |
10 µm |
712210.10 |
712210.100 |
| NUCLEOSIL 120-7 NH2 |
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~ 2 % |
120 Å |
7 µm |
712610.10 |
712610.100 |
| NUCLEOSIL 300-7 NH2 |
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~ 2 % |
300 Å |
7 µm |
712919.10 |
712919.100 |
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