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Instruction leaflet:
NUCLEOSIL®
NH2 / NH2-RP


  
   
NUCLEOSIL® NH2 / NH2 RP
Amino modified silica phases for HPLC · USP L8
Features
Polar aminopropyl modified silica for reversed phase and normal phase chromatography:
Normal phase chromatography with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
Reversed phase chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
Anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile)
Available pore sizes 100, 120 and 300 Å, available particle sizes 3 µm, 5 µm, 7 µm and 10 µm,
pH stability at 20 °C: 2–8, carbon content 3.5 %
Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions
Corresponding NUCLEODUR® phase see NUCLEODUR® NH2 / NH2-RP
For numerous applications on NUCLEOSIL® NH2 phases click here:


EC standard columns for analytical HPLC
Ordering information
Eluent in column (except for NUCLEOSIL® 100-5 NH2 RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.
ID Particle size Pore size   Length 250 mm   EC guard
columns*
CC guard
columns**
NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile – water)    
4.6 mm 5 µm 100 Å   720095.46RP   721155.30 721605.40RP
NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane)    
4.6 mm 5 µm 100 Å   720095.46   721020.30 721605.40
NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane )    
4.6 mm 10 µm 100 Å   720025.46      
Columns/pack ->   1   3 3
* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359).
Custom-packed EC columns with other dimensions are available on request.


NUCLEOSIL® NH2 bulk packings

Ordering information
Phase   Carbon content Pore size Particle Size Pack of 10 g Pack of 100 g
NUCLEOSIL 100-5 NH2   3.5 % 100 Å 5 µm 712200.10 712200.100
NUCLEOSIL 100-10 NH2   3.5 % 100 Å 10 µm 712210.10 712210.100
NUCLEOSIL 120-7 NH2   ~ 2 % 120 Å 7 µm 712610.10 712610.100
NUCLEOSIL 300-7 NH2   ~ 2 % 300 Å 7 µm 712919.10 712919.100
 

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