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Silylation reagents
MSTFA · MSHFBA · MBDSTFA |
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Features
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N-methyl-N-trimethylsilyl-trifluoroacetamide
m.w. 199.1, Bp 70 °C (75 mm Hg), density d20°/4° = 1.11 |
MSTFA:
R' = CF3, R" = CH3 |
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The most volatile trimethylsilyl amide available
Very strong TMS donor which does not cause any noticeable FID fouling even after long-time measuring series
The already good solution characteristics can be improved by addition of submolar quantities of protic solvents (e.g., TFA for extremely polar compounds such as hydrochlorides) or pyridine (e.g., for carbohydrates).
Recommended application: carboxylic acids, hydroxy and ketocarboxylic acids, amino acids, amines, alcohols, polyalcohols, sugars, mercaptans and similar compounds with active hydrogen atoms. Even amine hydrochlorides can be silylated directly.
Advantages: complete reaction with high reaction rates, even without a catalyst (1–2 % TMCS or TSIM)
The by-product of the reaction (N-methyltrifluoroacetamide) features high volatility and short retention time.
For a working protocol click here. |
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N-methyl-N-trimethylsilyl-heptafluorobutyramide
m.w. 299.1, Bp 148 °C (760 mm Hg) |
MSHFBA:
R' = C3F7, R" = CH3 |
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Similar to MSTFA in reactivity and chromatography
Recommended application: carboxylic acids, alcohols, phenols, primary and secondary amines and amino acids
Used either alone or in combination with a catalyst (TMCS, TSIM) or another silylation reagent with or without solvent
The by-product N-methylheptafluorobutyric amide has a lower retention time than the silylating reagent.
Especially useful for flame ionisation detection due to the large ratio of fluorine to silicon of 7:1, since degradation of the excess of MSHFBA does not produce SiO2 but volatile, non-corrosive silicon compounds
For a working protocol click here. |
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N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide
m.w. 241.3, Bp 168–170 °C (760 mm Hg), density d20°/4° = 1.121 |
MBDSTFA:
R' = CF3, R" = C4H9 |
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Silylation reagent which donates a tert-butyldimethylsilyl group (TBDMS) for derivatizing active hydrogen atoms in hydroxyl, carboxyl and thiol groups as well as primary and secondary amines
Fast reactions (typically 5–20 min) with high yields (> 96 %)
By-products are neutral and volatile.
TBDMS ethers are 104 times more stable than the corresponding TMS ethers; chromatographic retention times are longer due to the large protecting group, which may improve some separations;
because of the high molecular ion concentration at M+-57 useful for GC-MS applications
For a working protocol click here. |
Ordering information
| Packing unit |
MSHFBA |
MSTFA |
MBDSTFA |
| 10 x 1 mL |
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701440.101 |
| 20 x 1 mL |
701260.201 |
701270.201 |
701440.201 |
| 1 x 10 mL |
701260.110 |
701270.110 |
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| 5 x 10 mL |
701260.510 |
701270.510 |
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| 1 x 100 mL |
701260.1100 |
701270.1100 |
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| 6 x 50 mL |
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701270.650 |
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| 6 x 100 mL |
701260.6100 |
701270.6100 |
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| 12 x 100 mL |
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701270.12100 |
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Due to their purpose, derivatization reagents are very reactive
substances. For this reason they should be stored cool and protected
from moisture. Our derivatization reagents are supplied in vials with
crimp caps for easy access with a syringe. Vials with pierced sealing
disks have limited stability and should be used soon. |
| These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS. |
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