Silylation reagents MSTFA · MSHFBA · MBDSTFA
Features

	N-methyl-N-trimethylsilyl-trifluoroacetamide m.w. 199.1, Bp 70 °C (75 mm Hg), density d20°/4° = 1.11	MSTFA: R' = CF3, R" = CH3
	the most volatile trimethylsilyl amide available very strong TMS donor which does not cause any noticeable FID fouling even after long-time measuring series The already good solution characteristics can be improved by addition of submolar quantities of protic solvents (e. g. TFA for extremely polar compounds such as hydrochlorides) or pyridine (e. g. for carbohydrates). recommended application: carboxylic acids, hydroxy and ketocarboxylic acids, amino acids, amines, alcohols, polyalcohols, sugars, mercaptans and similar compounds with active hydrogen atoms. Even amine hydrochlorides can be silylated directly. advantages: complete reaction with high reaction rates, even without a catalyst (1–2  % TMCS or TSIM) the by-product of the reaction (N-methyltrifluoroacetamide) features high volatility and short retention time For a working protocol click here.
	N-methyl-N-trimethylsilyl-heptafluorobutyramide m.w. 299.1, Bp 148 °C (760 mm Hg)	MSHFBA: R' = C3F7, R" = CH3
	similar to MSTFA in reactivity and chromatography recommended application: carboxylic acids, alcohols, phenols, primary and secondary amines and amino acids used either alone or in combination with a catalyst (TMCS, TSIM) or another silylation reagent with or without solvent the by-product N-methylheptafluorobutyric amide has a lower retention time than the silylating reagent  especially useful for flame ionisation detection due to the large ratio of fluorine to silicon of 7 : 1, since degradation of the excess of MSHFBA does not produce SiO2 but volatile, non-corrosive silicon compounds For a working protocol click here.
	N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide m.w. 241.3, Bp 168 – 170 °C (760 mm Hg), density d20°/4° = 1.121	MBDSTFA: R' = CF3, R" = C4H9
	silylation reagent which donates a tert-butyldimethylsilyl group (TBDMS) for derivatising active hydrogen atoms in hydroxyl, carboxyl and thiol groups as well as primary and secondary amines fast reactions (typically 5 – 20 min) with high yields (> 96%) by-products are neutral and volatile TBDMS ethers are 104 times more stable than the corresponding TMS ethers; chromatographic retention times are longer due to the large protecting group, which may improve some separations; because of the high molecular ion concentration at M+-57 useful for GC-MS applications For a working protocol click here.

Ordering information

Packing unit	MSHFBA	MSTFA	MBDSTFA
10 x 1 ml			701440.101
20 x 1 ml	701260.201	701270.201	701440.201
1 x 10 ml	701260.110	701270.110
5 x 10 ml	701260.510	701270.510
1 x 100 ml	701260.1100	701270.1100
6 x 50 ml		701270.650
6 x 100 ml	701260.6100	701270.6100
12 x 100 ml		701270.12100
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture. Our derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used soon.