Silylation reagents BSA · BSTFA
Features

	N,O-bis-trimethylsilyl-acetamide m.w. 203.4, Bp 71 – 73 °C (35 mm Hg), density d20°/4° = 0.832	BSA: R = CH3
	strong silylation reagent, which forms very stable TMS derivatives of a wide variety of compounds, e. g. alcohols, amines, carboxylic acids, phenols, steroids, biogenic amines and alkaloids not recommended for use with carbohydrates or very low molecular weight compounds good solvent for polar compounds, but frequently used in combination with a solvent (pyridine, DMF etc.) or with other silylation reagents. When used with DMF, BSA is the reagent of choice for derivatising phenols. For a working protocol click here.
	N,O-bis-trimethylsilyl-trifluoroacetamide m.w. 257.4, Bp 40 °C (12 mm Hg), density d20°/4° = 0.961	BSTFA: R = CF3
	powerful trimethylsilyl donor with approximately the same donor strength as the nonfluorinated analog BSA advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids. For a working protocol click here. BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1 % trimethylchlorosilane (TMCS), available under the designation SILYL-991. For a working protocol click here.

Ordering information

	Packing unit
Code	20 x 1 ml	1 x 10 ml	5 x 10 ml	1 x 50 ml	1 x 100 ml
BSA		701210.110	701210.510	701210.150
BSTFA	701220.201	701220.110	701220.510
SILYL-991 (BSTFA - TMCS 99:1)	701490.201			701490.150	701490.1100
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture. Our derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used soon.