Editors
Silylation reagents BSA · BSTFA
Features
N,O-bis-trimethylsilyl-acetamide
M 203.4 g/mol, Bp 71–73 °C (35 mm Hg), density d20°/4° = 0.832
BSA:
R = CH3
  Strong silylation reagent, which forms very stable TMS derivatives of a wide variety of compounds, e.g., alcohols, amines, carboxylic acids, phenols, steroids, biogenic amines and alkaloids
Not recommended for use with carbohydrates or very low molecular weight compounds
Good solvent for polar compounds, but frequently used in combination with a solvent (pyridine, DMF etc.) or with other silylation reagents
When used with DMF, BSA is the reagent of choice for derivatizing phenols. For a working protocol click here.
 
N,O-bis-trimethylsilyl-trifluoroacetamide
M 257.4 g/mol, Bp 40 °C (12 mm Hg), density d20°/4° = 0.961
BSTFA:
R = CF3
  Powerful trimethylsilyl donor with approximately the same donor strength as the nonfluorinated analog BSA
Advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids) – For a working protocol click here.
BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1 % trimethylchlorosilane (TMCS), available under the designation SILYL-991. For a working protocol click here.


Ordering information
  Packing unit
Substance 20 x 1 mL 1 x 10 mL 5 x 10 mL 1 x 50 mL 1 x 100 mL
BSA   701210.110 701210.510 701210.150  
BSTFA 701220.201 701220.110 701220.510    
SILYL-991
(BSTFA – TMCS 99:1)
701490.201     701490.150 701490.1100
Due to their purpose, derivatization reagents are very reactive substances. For this reason they should be stored cool and protected from moisture. Our derivatization reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used soon.
These products contain harmful substances which must be specially labeled as hazardous. For detailed information please see MSDS.

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