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Acylation reagents for GC
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Acyl halides
by-product of acylation with acyl halides: corresponding hydrohalic acids
excess of reagent and acid have to be removed or trapped by a suitable base (e. g. pyridine)
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Pentafluorobenzoyl chloride
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m.w. 230.52, Bp 158 – 159 °C (760 mm Hg),
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PFBC:
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R = C6F5, X = Cl
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density d20°/4° = 1.601
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Anhydrides
by-products of acylation with anhydrides: corresponding acids
excess reagent and the acid formed have to be removed
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Acylation with fluorinated acid anhydrides can be used for alcohols, phenols, carboxylic acids, amines, amino acids and steroids forming volatile, stable derivatives suited for FID as well as for ECD detection. For working protocols click here: TFAA / HFBA
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Trifluoroacetic acid anhydride
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m.w. 210.04, Bp 39.5 – 40.5 °C (760 mm Hg),
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TFAA:
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R = CF3
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density d20°/4° = 1.490
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Heptafluorobutyric acid anhydride
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m.w. 410.06, Bp 106 – 107 °C (760 mm Hg),
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HFBA:
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R = C3F7
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density d20°/4° = 1.665
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Bisacylamides
by-products: corresponding neutral acylamides, which can be easily removed due to their high volatility; because of neutral conditions and favourable chromatographic properties often removal of the bisacylamide is not necessary. Thus sample preparation is much more convenient.
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Acylation with fluorinated acid amides is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions. MBTFA also forms very volatile derivatives with carbohydrates. For working protocols click here: MBTFA / MBHFBA
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N-methyl-bis(trifluoroacetamide)
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m.w. 223.08, Bp 123 – 124 °C (760 mm Hg),
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MBTFA:
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R = CF3
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density d20°/4° = 1.55
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N-methyl-bis(heptafluorobutyramide)
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m.w. 423.1, Bp 165 – 166 °C (760 mm Hg),
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MBHFBA:
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R = C3F7
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density d20°/4° = 1.673
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Ordering information
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Packing unit
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Code
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10 x 1 ml
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20 x 1 ml
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1 x 10 ml
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5 x 10 ml
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HFBA
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701110.201
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701110.110
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701110.510
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MBTFA
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701410.201
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701410.110 |
701410.510 |
MBHFBA
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701420.101 |
701420.201 |
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PFBC
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701120.101 |
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TFAA
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701130.110 |
701130.510 |
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture. Our derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used soon.
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