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| Three steps to open/download MSDS |
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Select your language |
| 2 |
Select the folder with the first 4 digits of the REF No. (e.g. 7011 for HFBA) |
| 3 |
Data sheets are displayed according to REF No. and can be opened or saved. |
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| Derivatisation reagents for GC |
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| Purpose of derivatisation |
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improved volatility, better thermal stability or a lower limit of detection in GC |
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prerequisite: quantitative, rapid and reproducible formation of only one derivative |
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halogen atoms introduced by derivatisation (e. g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity |
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elution orders and fragmentation patterns in MS can be influenced by a specific derivatisation |
Derivatisation method development kits
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Designation / purpose |
Contents of the kit |
REF |
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Derivatisation method development kit |
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which type of derivatisation is best suited for
your sample (alkylation, acylation or silylation)? |
2 x 1 ml each of TMSH, MSTFA, MBTFA |
701952 |
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Acylation kit |
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which is the proper reagent for acylation? |
2 x 1 ml each of MBTFA, TFAA, MBHFBA |
701950 |
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Alkylation kit |
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which is the proper reagent for methylation? |
3 x 1 ml each of TMSH, DMF-DMA |
701951 |
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Silylation kit |
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which is the proper reagent for silylation? |
2 x 1 ml each of MSTFA, BSTFA, TSIM, MSHFBA |
701953 |
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Selection guide for derivatisation of important functional groups in GC
| Function |
method |
derivative |
recommended reagents |
Alcohols,
Phenols
R'OH |
silylation |
R'O - TMS |
BSA, MSTFA, MSHFBA, TSIM,
SILYL‑2110, SILYL-21, SILYL-1139 |
| acylation |
R'O - CO - R |
TFAA, HFBA, MBTFA, MBHFBA |
| alkylation |
R'O - R |
TMSH |
| sterically hindered |
silylation |
R'O - TMS |
TSIM, BSTFA, SILYL-991 |
Amines
primary, secondary |
silylation |
R' - NR" - TMS |
BSA, MSTFA, MSHFBA, SILYL-991 |
| acylation |
R' - NR" - CO - R |
TFAA, HFBA, MBTFA, MBHFBA |
| hydrochlorides |
silylation |
R' - NR" - TMS |
MSTFA |
| Amides |
silylation |
not stable |
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| acylation |
R' - CO - NH - CO - R |
TFAA, MBTFA, HFBA, MBHFBA |
| Amino acids |
silylation |
R' – CH(NH – TMS) – CO – O – TMS |
BSA, BSTFA, MSTFA, MSHFBA |
alkylation (a)
+ acylation (b) |
R' – CH(NH - CO-R) – CO – O – R |
a) MeOH/TMCS, TMSH
b) TFAA, HFBA, MBTFA, MBHFBA |
Carboxylic acids
(fatty acids) |
silylation |
R’ – CO – O – TMS
susceptible to hydrolysis |
BSA, MSTFA, MSHFBA, TMCS, TSIM,
SILYL-2110, SILYL-21, SILYL-1139 |
| alkylation |
R’ – CO – O – R |
DMF-DMA, MeOH/TMCS (1 M), TMSH |
| salts |
silylation |
R’ – CO – O – TMS
susceptible to hydrolysis |
TMCS |
| Carbohydrates |
silylation |
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MSTFA, TSIM, HMDS, SILYL-1139 |
| acylation |
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TFAA, MBTFA |
| Steroids |
silylation |
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BSA, TSIM |
| acylation |
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TFAA, MBTFA, HFBA, MBHFBA |
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