Cellulose MN 300 DEAE
DEAE-modified cellulose ion exchange layers for TLC
Features

	fibrous cellulose modified with diethylaminoethyl groups: R - O - C2H4 - N(C2H5)2 available as POLYGRAM polyester sheets without fluorescent indicator
	mixed layers of cellulose MN 300 DEAE and high purity cellulose MN 300 HR are recommended for separation of mono- and oligonucleotides in nucleic acid hydrolysates

Separation of mono- and oligonucleotides in nucleic acid hydrolysates on layers of MN 300 DEAE/HR
The Medical Research Council Laboratory of Molecular Biology in Cambridge (UK) has developed a special procedure for the separation of radioactively labelled mono- and oligonucleotides in hydrolysates of ribonucleic acid. It is a 2-dimensional procedure, in which mononucleotides and oligonucleotides are separated up to n = 50. The separation process consists of 2 stages, first a high voltage electrophoretic group fractionation on acetate sheets in the 1st dimension and then a TLC separation in the 2nd dimension after blotting of the preseparated substances onto a mixed layer of DEAE cellulose and HR cellulose in the ratio 2:15.		As eluent concentrated urea solutions with addition of homomix solutions are used, which consist of ribonucleic acid hydrolysates and dialysates. Mononucleotides move up to the front, and depending on chain length the oligonucleotides appear between the Rf values 1 and 0. The evaluation of chromatograms is by autoradiography after treatment with red ink, which contains radioactive sulphur 35S. References G. G. Brownlee et al., European J. Biochem. 11 (1969) 395 B. E. Griffin, FEBS Letters 15 (1971) 165 F. Sanger et al., J. Mol. Biol. 13 (1965) 373 – 398.

Ordering information

Designation	Thickness of layer	Plate size	Fluorescent indicator	Plates per pack	Cat. No.
POLYGRAM® polyester sheets
CEL 300 DEAE	0.10 mm	5 x 20 cm	–	50	801072
CEL 300 DEAE	0.10 mm	20 x 20 cm	–	25	801073
CEL 300 DEAE	0.10 mm	40 x 20 cm	–	25	801074
CEL 300 DEAE/HR-2/15	0.10 mm	40 x 20 cm	–	25	801084