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Nano-SIL CN
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cyano-modified nano silica layers for HPTLC
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Features
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base material:
silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å, specific pore volume 0.75 ml/g,
particle size 2 – 10 μm, pH stability 2 – 10
indicator:
acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm); UV-absorbing substances apppear as dark-blue to black spots on a light-blue background
cyanopropyl modification, carbon content 5.5%
order of polarity:
silica > DIOL > NH2 > CN > RP-2 > C 18-50 > RP-18 W > C 18-100
available as glass plates or ALUGRAM aluminium sheets
normal phase or reversed phase separation modes depending on the polarity of the developing solvent (see figure below)
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recommended application: steroid hormones, phenols, preservatives |
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Retention values of steroids as function of eluent composition
Layer: Nano-SIL CN/UV
Polarity of the eluent governs the type of separation mechanism:
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eluent system petroleum ether (PE) - acetone
(NP mode):
the higher the concentration of PE, the stronger are the adsorptive interactions of the steroids with the stationary phase |
eluent system acetone – water
(RP mode):
the sequence of elution of the steroids is reversed, the most nonpolar compounds are most strongly retained |
Ordering information
Designation
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Thickness of layer |
Plate size
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Fluorescent indicator
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Plates per pack
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Cat. No.
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Glass plates
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| Nano-SIL CN/UV |
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0.20 mm |
10 x 10 cm
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UV254 |
25
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811115
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| Nano-SIL CN/UV |
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0.20 mm |
10 x 20 cm
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UV254 |
25
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811116
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ALUGRAM® aluminium sheets
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| Nano-SIL CN/UV |
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0.15 mm |
4 x 8 cm
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UV254 |
50
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818184 |
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| Nano-SIL CN/UV |
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0.15 mm |
20 x 20 cm
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UV254 |
25
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818185
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