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| Nano-SIL CN |
Glass
plates |
ALUGRAM®
sheets |
| Cyano-modified HPTLC silica layers |
Features
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Base material:
Nano silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å, specific pore volume 0.75 mL/g,
particle size 2–10 μm, pH stability 2–8
Indicator:
acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm)
UV-absorbing substances apppear as dark-blue to black spots on a light-blue background.
Cyanopropyl modification, carbon content 5.5 %
Order of polarity:
silica > DIOL > NH2 > CN > RP-2 > C18-50 > RP-18 W > C18-100
Available as glass plates or ALUGRAM® aluminium sheets
Normal phase or reversed phase separation modes depending on the polarity of the developing solvent (see figure below) |
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Recommended application: steroid hormones, phenols, preservatives
For applications click here. |
Retention values of steroids as function of eluent composition
Layer: Nano-SIL CN/UV
Polarity of the eluent governs the type of separation mechanism: |
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Eluent system petroleum ether (PE) - acetone
(NP mode):
the higher the concentration of PE, the stronger are the adsorptive interactions of the steroids with the stationary phase |
Eluent system acetone – water
(RP mode):
the sequence of elution of the steroids is reversed, the most nonpolar compounds are most strongly retained |
Ordering information
| Designation |
Thickness of layer |
Plate size |
Fluorescent indicator |
Plates per pack |
REF |
| Glass plates |
| Nano-SIL CN/UV |
0.20 mm |
10 x 10 cm |
UV254 |
25 |
811115 |
| Nano-SIL CN/UV |
0.20 mm |
10 x 20 cm |
UV254 |
25 |
811116 |
| ALUGRAM® aluminium sheets |
| Nano-SIL CN/UV |
0.15 mm |
4 x 8 cm |
UV254 |
50 |
818184 |
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