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Nano-SIL NH2
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Influence of eluent composition on the separation of nuceotides

Layer: Nano-SIL NH2/UV
Eluent: MeOH – water according to figure + 0.18 M NaCl
Migration distance: 7 cm
c-AMP, AMP: adenosine monophosphate
ADP: adenosine diphosphate
ATP: adenosine triphosphate
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amino-modified nano silica layers for HPTLC
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Features
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base material:
silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å, specific pore volume 0.75 ml/g, particle size 2 – 10 μm,
pH stability 2 – 10
indicator:
acid-resistant product with a pale blue fluorescence for short-wave UV
(254 nm); UV-absorbing substances apppear as dark-blue to black spots on a light-blue background
aminopropyl modification, carbon content 3.5%
order of polarity:
silica > DIOL > NH2 > CN > RP-2 > C 18-50 > RP-18 W > C 18-100
available with or without fluorescent indicator, as glass plates or ALUGRAM aluminium sheets
layer can be wetted equally well by pure water as by organic solvents |
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recommended application: vitamins, sugars, steroids, purine derivatives, xanthines, phenols, nucleotides and pesticides |
Ordering information |
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Designation
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Thickness of layer |
Plate size
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Fluorescent indicator
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Plates per pack
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Cat. No.
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Glass plates
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| Nano-SIL NH2 |
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0.20 mm |
10 x 10 cm
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25
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811109
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| Nano-SIL NH2/UV |
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0.20 mm |
10 x 10 cm
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UV254 |
25
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811111
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| Nano-SIL NH2/UV |
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0.20 mm |
10 x 20 cm
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UV254 |
25
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811112
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ALUGRAM® aluminium sheets
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| Nano-SIL NH2/UV |
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0.15 mm |
4 x 8 cm
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UV254 |
50
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818182 |
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| Nano-SIL NH2/UV |
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0.15 mm |
20 x 20 cm
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UV254 |
25
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818183
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