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User manual NucleoSpin®
Plant II

  
     

NucleoSpin® Plant II

Genomic DNA from plant and fungi

Select the most efficient lysis buffer

Features 

The second generation for rapid isolation of genomic DNA from plant samples
with higher yield and quality

 

• Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
   Lysis Buffer PL1, based on CTAB lysis method
   Lysis Buffer PL2, based on SDS lysis method
• NucleoSpin® Filters included for clarification of lysate
• Improved buffer compositions → higher yield and purity
• Optimized silica membrane → improved DNA binding
• RNase A included

 

Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 100 mg plant tissue (wet weight), < 20 mg plant tissue (dry weight)

Lysate clarification NucleoSpin® Filters
Fragment size 50 bp–approx. 50 kbp
Typical yield 1–30 µg
A260/A280 1.8–1.9
Elution volume 2 x 50 µL
Preparation time 30 min/prep
Binding capacity 50 µg

Applications

• DNA from plant cells and tissue
• DNA from fungi

• Typical downstream applications: PCR, Southern blotting, enzymatic reactions

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® Plant II

10

NucleoSpin Plant® II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A 740770.10

50

as above 740770.50

250

as above 740770.250

For ordering click here

  
     
  
   
  
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