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User Manual NucleoSpin
Plant II

  
     

NucleoSpin® Plant II

Genomic DNA from plant and fungi

Features 
The new generation for rapid isolation of genomic DNA from plant samples with higher yield and quality

• Two alternative lysis buffers included for optimal processing of various samples
   and highest flexibility
   Lysis Buffer PL1, based on CTAB lysis method
   Lysis Buffer PL2, based on SDS lysis method

• NucleoSpin Filters included for clarification of lysate
• Improved buffer compositions ® higher yield and purity
• Optimized silica membrane ® improved DNA binding
• RNase A included

  
Technology Silica-membrane technology
Format Mini spin columns
Sample material

<100 mg plant tissue (wet weight), <20 mg plant tissue (dry weight)

Lysate clarification NucleoSpin Filters
Fragment size 50 bp- >30 kbp
Typical yield 1-30 µg
A260/280 1.6-1.9
Elution volume 2 x 50 µl
Preparation time 30 min/prep
Binding capacity 50 µg
Applications

• DNA from plant cells and tissue
• DNA from fungi
• DNA suitable for e.g. PCR, Southern blotting, enzymatic reactions

  
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Ordering information

Product

Preps

Specification Reference
NucleoSpin Plant II

10

NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes (2 ml), buffers, RNase A 740770.10

50

as above 740770.50

250

as above 740770.250

For ordering click here

  
     
  
     
  
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