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NucleoBond® Xtra Midi / Maxi

Transfection-grade plasmid DNA

Blue LyseControl –
efficient alkaline lysis

Features
The new generation of anion exchanger – ultra-fast, transfection-grade plasmid DNA with high yield

NucleoBond Xtra Midi Column

• Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
• Column filter included – high filter flow rates, parallel lysate clearing, and loading onto the column
• Improved silica material – typical yields of 250 μg (Midi) and 1 000 μg (Maxi)
• Proven anion-exchange technology – transfection-grade plasmid DNA
• NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation


NucleoBond® Xtra Midi NucleoBond® Xtra Maxi
Technology Anion-exchange chromatography Anion-exchange chromatography
Format Midi gravity flow columns Maxi gravity flow columns
Lysate clarification Column filters Column filters
Sample material < 200 mL (high copy)
< 400 mL (low copy)
< 600 mL (high copy)
< 1200 mL (low copy)

Vector size

< 300 kbp < 300 kbp
Typical yield 400 µg 1000 µg
A260/A280 1.80–1.95 1.80–1.95
Isopropanol precipitation

Xtra Midi

Centrifugation

Xtra Midi Plus

NucleoBond® Finalizer

Xtra Maxi

Centrifugation

Xtra Maxi Plus

NucleoBond® Finalizer Large

Preparation time 70 min/prep 30 min/prep 75 min/prep 35 min/prep

 

Applications
• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required, see "Ordering information")

 

  
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Ordering information

Product

Preps

Specification REF
NucleoBond® Xtra Midi kits


NucleoBond® Xtra Midi

10

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740410.10

50

as above 740410.50

100

as above 740410.100
NucleoBond® Xtra Midi Plus

10

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers, buffers, RNase A

740412.10

50

as above 740412.50
NucleoBond® Xtra Maxi kits


NucleoBond® Xtra Maxi

10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740414.10

50

as above 740414.50

100

as above 740414.100
NucleoBond® Xtra Maxi Plus

10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers Large, buffers, RNase A

740416.10

50

as above 740416.50
Product accessories

Pack of

Specification REF
NucleoBond® Xtra Combi Rack

1

for use with NucleoBond® Xtra Midi, Maxi, BAC, AX 100, 500, 2000, and 10000, BAC 100 Columns 740415
NucleoBond® Xtra Buffer Set I

1

for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra kits, sufficient for 10 Xtra Maxi preps or 20 Xtra Midi preps. Set includes 150 mL buffers RES, LYS, NEU, and RNase A
740417

For ordering click here

  
     

NEWS

NucleoBond®
Xtra
Midi / Maxi

LyseControl
now included

 

  
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