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NucleoSpin® RNA/Protein

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NucleoSpin® RNA/Protein 

  
     
NucleoSpin® RNA/Protein

RNA and protein isolation

Features
Parallel isolation of RNA and protein from undivided samples
• No splitting of samples required – reliable interpretation of RNA and protein extracted
   from the same sample (direct correlation)
• High RNA yield and integrity
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete mini kit with NucleoSpin® Filters (shredders) and recombinant DNase for
   DNA-free RNA of high quality
Technology Silica-membrane technology
Format Mini spin columns
Sample material < 5 x 106 cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue

RNA Protein
Fragment size > 200 nt

15–300 kDa

Typical yield < 70 µg

< 1200 µg

A260/A280 1.9–2.1

Typical RIN (RNA integrity number) > 9

Elution volume (RNA)
Resolubilization volume (protein)
40–120 µL

10–100 µL

Preparation time 30 min/6 preps

35 min/6 preps

Binding capacity 200 µg

Applications*

• Rapid purification of total RNA and protein from cultured cells and tissue
• Gene expression profiling, siRNA experiments, analysis of transgenic organisms, drug screening
• Broad spectrum of starting material tested and proteins already detected
Starting materials successfully used
Proteins successfully analyzed
In general: cultured cells / fresh tissue / frozen tissue / tissue in RNAlater® / plants
In detail: cortex of kidney / medulla tissue / liver tissue / neonatal rat cardiomyocytes / macrophages / HeLa / HEK 239 / MCF-7 / D14 SJR / D05 GSM 1A / T47D / 293 / U251 / U373 / HCT116 / GaMG / VH6-TE / garden cress

 

In general: cytosolic proteins / membrane proteins / phosphorylated proteins / lipoproteins / glycoproteins / small proteins (17 kDa) / large proteins (250 kDa)
In detail: Cyclin D1 / p53 / Cleaved Casp-3 / Bax / Erk2 / RNA binding protein / β-Actin / α-Actinin-4 / phospho-ERK1/2 / total-ERK1/2 / γ-Tubulin / MMP1 (active) / pro-MMP / E-Cadherin / prion protein / AMP activated kinase (AMPK)

* Kits to be used for research purposes only
  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA/Protein

10

NucleoSpin® RNA/Protein Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes
(1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
740933.10

50

as above 740933.50

250

as above 740933.250

For ordering click here

  
   
  
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