Protino 96 Ni-IDA

Protino^® 96 Ni-IDA

Purification of His-tag proteins

Features

Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins

• Chelating group IDA (iminodiacetic acid), three protein binding sites
• High protein yield/recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins
compared to other common IMAC matrices

• Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin
® storage at room temperature
• Buffers included

Technology
IMAC (immobilized metal ion affinity chromatography)

Chelating group
IDA (iminodiacetic acid)

Format
ready-to-use 96-well plates

Matrix
Macroporous silica

Physical form

Dry matrix, precharged with Ni²⁺

Amount of resin per well

50 mg

Bed volume
100 µL

Binding capacity*
1 mg/well

* refers to 6xHis-GFPuv

Applications

• Purification of polyhistidine-tagged proteins
• 96-well format gravity flow chromatography