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Protino® Ni-IDA Resin
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Purification of His-tag proteins
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| Features |
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| Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins |
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• Chelating group IDA (iminodiacetic acid), three protein binding sites
(also see Technologies)
• High protein yield / recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins
compared to other common IMAC matrices
• Dry resin → storage at room temperature
Protino® Ni-IDA Resin
• Dry bulk material
• Rigid support silica matrix, compatible with high pressure and high flow rates,
does not shrink or swell → highest flexibility
• Batch procedure, gravity-flow column chromatography, FPLC™ applications
• Empty Protino® columns available for use with Protino® Ni-IDA Resin
• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
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| Technology |
IMAC, immobilized metal ion affinity chromatography
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| Chelating ligand |
IDA (iminodiacetic acid)
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| Format |
Bulk matrix
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| Matrix |
Macroporous silica
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Physical form
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Dry matrix, precharged with Ni2+
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Binding capacity*
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20 mg/g resin
10 mg/mL bed volume
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| Max. pressure |
145 psi (10 bar)
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| * Binding capacity will vary for each polyhistidin-tagged protein |
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| Applications |
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• Purification of polyhistidine-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications |
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Ordering information
| Product |
Preps
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Specification |
REF |
| Protino® Ni-IDA Resin |
5 g
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745210.5 |
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30 g
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745210.30 |
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120 g
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745210.120 |
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600 g
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745210.600 |
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| Product accessories |
Pack of
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Specification |
REF |
| Protino® Columns 14 mL |
10
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Empty columns for use with e.g. Protino® Ni-TED / IDA Resin |
745250.10 |
| Protino® Columns 35 mL |
10
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Empty columns for use with e.g. Protino® Ni-TED / IDA Resin |
745255.10 |
| Receiver Plate 50 µm hydrophilized |
4
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96-well plates with an inserted hydrophilized filter frit of 50 µm pore size, suitable for gravity flow, centrifugation, and vacuum |
740689.4 |
For ordering click here
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