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Protino® Ni-IDA Packed Columns
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Purification of His-tag proteins
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| Features |
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| Protino Ni-IDA, the purer alternative for higher yields of His-tag proteins |
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 • Chelating group IDA, three protein binding sites
• High protein yield/recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins
compared to other common IMAC matrices
• Ready-to-use prepacked columns filled with dry Protino Ni-IDA Resin
® storage at room temperature
• Buffers included
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Protino Ni-IDA
150 packed columns
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Protino Ni-IDA
1000 packed columns
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Protino Ni-IDA
2000 packed columns
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| Technology |
IMAC (immobilized metal ion affinity chromatography)
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| Chelating ligand |
IDA (iminodiacetic acid)
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| Format |
Mini gravity flow
columns
filled with
Protino Ni-IDA Resin
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Midi gravity flow
columns
filled with
Protino Ni-IDA Resin
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Maxi gravity flow
columns
filled with
Protino Ni-IDA Resin
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| Matrix |
Macroporous silica
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| Physical form |
Dry matrix, precharged with Ni2+
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Amount of resin
per column
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40 mg
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250 mg
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500 mg
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| Bed volume |
80 µl
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500 µl
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1000 µl
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| Binding capacity* |
800 µg
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5 mg
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10 mg
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| * refers to 6xHis-GFPuv |
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Applications
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| • Purification of polyhistidine-tagged proteins |
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Ordering information
| Product |
Preps
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Specification |
Reference |
| Protino Ni-IDA 150 Packed Columns |
10
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Protino Ni-IDA 150 Packed Columns, buffers |
745150.10 |
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50
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as above |
745150.50 |
| Protino Ni-IDA 1000 Packed Columns |
5
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Protino Ni-IDA 1000 Packed Columns, buffers |
745160.5 |
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50
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as above |
745160.50 |
| Protino Ni-IDA 2000 Packed Columns |
5
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Protino Ni-IDA 2000 Packed Columns, buffers |
745170.5 |
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25
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as above |
740170.25 |
For ordering click here
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