NucleoSpin® RNA/DNA Buffer Set	Total RNA and DNA isolation
Features
Parallel isolation of RNA and DNA from unsplitted samples in one working procedure
• To be used in combination with NucleoSpin RNA II, NucleoSpin RNA Plant, NucleoSpin RNA/Protein kits • No need to split samples (e.g. precious, indivisible samples like biopsy material) • High quality DNA and RNA from one sample, perfect for PCR, RT-PCR, real-time PCR • Fast procedure

  

Format	Buffer set for elution of DNA in combination with RNA preparations, to be used in combination with NucleoSpin RNA kits
Sample material	See NucleoSpin RNA II, NucleoSpin RNA Plant, NucleoSpin RNA/Protein
DNA size	£ 20 kbp
Typical DNA yield	5 µg from 106 HeLa cells 16 µg from 30 mg pig liver 5 µg from 100 mg maize leaf
A260/280	1.7-2.0
DNA elution volume	100 µl
RNA yield and purity	Identical to NucleoSpin RNA II, NucleoSpin RNA Plant, NucleoSpin RNA/Protein
Applications*
• For biopsy material: RNA for expression analysis; DNA for mutation analysis e.g. of cancer genes • For ticks (Ixodes ricinus): RNA for analysis of RNA viruses (e.g. TBE-V, Tick-borne encephalitis virus,    infectious agent of meningoencephalitis); DNA for analysis of germs (e.g. Borrelia burgdorferi, infective    agent of the Lyme disease) • For transgenic plants or animals: RNA for expression analysis of the transformed gene and/or other genes;    DNA for analysis of integration site, integration number and sequence confirmation of the transformed    gene • For transfected cultured cells: RNA for expression analysis of the transgene or other genes of interest;    DNA for analysis of methylation status of the transgene or other genes of interest
* KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.