Editors
     
  
     
Literature

User manual NucleoSpin® RNA Clean-up

  
     
NucleoSpin® RNA Clean-up

RNA clean-up

Features 
Simple, fast, and convenient clean-up of RNA

 

 

• Complete removal of RT-PCR inhibitors
• Time-saving procedure based on NucleoSpin® RNA, without DNase digestions and
   homogenization steps
• RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions
   (e.g. amplification reactions, labeling reactions)

Technology Silica-membrane technology
Format Mini spin columns
Sample material

200 µL phenol/chloroform extract, reaction mixture, or < 105 cells

Fragment size > 200 nt
Typical recovery 85–95%
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) Depending on sample quality (no significant loss of RIN detected)
Elution volume 40–120 µL
Preparation time 20 min/6 preps
Binding capacity 200 µg

Applications*

RNA clean-up of:
• Pre-purified RNA (e.g., Trizol®)
• Reaction mixtures
• Amino-allyl-mRNA
• Biotinylated RNA
• RNA isolation from up to 105 cultured cells (whenever co-purification of some genomic DNA is acceptable,
   kit does not contain rDNase)
• Typical downstream applications: enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip
   hybridizations
* Kits to be used for research purposes only
  
Maximize    

     

Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA Clean-up

10

NucleoSpin® RNA Clean-up Columns with Collection Tubes,  Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers 740948.10

50

as above 740948.50

250

as above 740948.250
Product accessories

Pack of

Specification REF
rDNase Set
click for detailed information

1

recombinant DNase and Reaction Buffer for rDNase, sufficient for 50 Mini preps of total RNA 740963

For ordering click here

  
   
  
Copyright 2017 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use