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User Manual NucleoSpin RNA Clean-up

  
     

NucleoSpin® RNA Clean-up

RNA clean-up

Features 
Simple, fast, and convenient clean-up of RNA

• Complete removal of RT-PCR inhibitors
• Time-saving procedure based on NucleoSpin RNA II, without DNase digestion and
   homogenization steps
• RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions
   (e.g. amplification reactions, labeling reactions, DNase digestions)
• Favourable price per prep

  
Technology Silica-membrane technology
Format Mini spin columns
Sample material

up to 200 µl phenol/chloroform extract or reaction mixture

Fragment size > 200 b
Typical recovery 85-95%
A260/280 1.9-2.1
Elution volume 40-100 µl
Preparation time 20 min/6 preps
Binding capacity 200 µg

Applications*

RNA clean-up of:
• Pre-purified RNA (e.g. Trizol®)
• Reaction mixtures
• Amino-Allyl-mRNA
• Biotinylated RNA
• RNA isolation from up to 105 cultured cells (whenever co-purification of some genomic DNA is acceptable,
   kit does not contain rDNase)
• RNA ready-to-use for e.g. enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip hybridizations

* Kits to be used for research purposes only

  
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Ordering information

Product

Preps

Specification Reference
NucleoSpin RNA Clean-up

10

NucleoSpin RNA II Columns with Collection Tubes,  Collection Tubes (2 ml), Collection Tubes (1.5 ml)buffers 740948.10

50

as above 740948.50

250

as above 740948.250
Product accessories

Pack of

Specification Reference
rDNase Set
click for detailed information

1

recombinant DNase and Reaction Buffer for rDNase, sufficient for 50 Mini preps of total RNA 740963

For ordering click here

  
     
  
     
  
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