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| NucleoSpin® Extract II |
PCR clean-up / Gel extraction
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15 µl elution volume ® highly concentrated DNA
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| Features |
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| PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume |
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• High recovery even for small fragments ³ 65 bp with efficient removal of
primers ® high efficiency
• Minimized elution volume: 15 µl ® highly concentrated DNA
• 10 min/6 preps ® time-saving procedure
• One buffer for PCR clean-up and gel extraction ® high convenience
• Suitable for all gel buffer systems (e.g. TAE, TBE)
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
<400 µl PCR reaction mixture
<400 mg TAE/TBE agarose gel
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| Fragment size |
65 bp-10 kbp |
| Typical recovery |
70-95% |
| A260/280 |
1.8-1.9 |
| Elution volume |
15-50 µl |
| Preparation time |
10 min/6 preps |
| Binding capacity |
15 µg |
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| Applications |
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• Purification of PCR products
• Extraction of DNA from agarose gels |
• Support protocol for concentration and desalination of reaction mixtures
• Support protocol for clean-up of single stranded DNA
(Binding Buffer NTC required, not included in the kit, see "Ordering information")
• Support protocol for clean-up of SDS-containing samples
(Binding Buffer NTB required, not included in the kit, see "Ordering information")
• DNA suitable for e.g. cloning, sequencing, PCR, restriction analysis |
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Ordering information
| Product |
Preps
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Specification |
Reference |
| NucleoSpin Extract II |
10
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NucleoSpin Extract II Columns,
Collection Tubes (2 ml), buffers |
740609.10 |
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50
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as above |
740609.50 |
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250
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as above |
740609.250 |
| Product accessories |
Pack of
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Specification |
Reference |
| Buffer NTB |
150 ml
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for clean-up of SDS-containing samples |
740595.150 |
| Buffer NTC |
100 ml
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for clean-up of single stranded DNA |
740654.100 |
For ordering click here
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