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NucleoSpin
Extract II

Product Flyer NucleoSpin Extract II Flyer

  
     

NucleoSpin® Extract II

PCR clean-up / Gel extraction

15 µl elution volume ® highly concentrated DNA

Features 
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• High recovery even for small fragments ³ 65 bp with efficient removal of
   primers ® high efficiency
• Minimized elution volume: 15 µl ® highly concentrated DNA
• 10 min/6 preps ® time-saving procedure
• One buffer for PCR clean-up and gel extraction ® high convenience
• Suitable for all gel buffer systems (e.g. TAE, TBE)

  
Technology Silica-membrane technology
Format Mini spin columns
Sample material

<400 µl PCR reaction mixture
<400 mg TAE/TBE agarose gel

Fragment size 65 bp-10 kbp
Typical recovery 70-95%
A260/280 1.8-1.9
Elution volume 15-50 µl
Preparation time 10 min/6 preps
Binding capacity 15 µg
Applications
• Purification of PCR products
• Extraction of DNA from agarose gels
• Support protocol for concentration and desalination of reaction mixtures
• Support protocol for clean-up of single stranded DNA 
   (Binding Buffer NTC required, not included in the kit, see "Ordering information")
• Support protocol for clean-up of SDS-containing samples
   (Binding Buffer NTB required, not included in the kit, see "Ordering information")
• DNA suitable for e.g. cloning, sequencing, PCR, restriction analysis

  
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Ordering information

Product

Preps

Specification Reference
NucleoSpin Extract II

10

NucleoSpin Extract II Columns,
Collection Tubes (2 ml), buffers
740609.10

50

as above 740609.50

250

as above 740609.250
Product accessories

Pack of

Specification Reference
Buffer NTB

150 ml

for clean-up of SDS-containing samples 740595.150
Buffer NTC

100 ml

for clean-up of single stranded DNA  740654.100

For ordering click here

  
     
  
     
  
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