NucleoSpin Gel and PCR Clean-up

NucleoSpin^® Gel and PCR Clean-up

PCR clean-up / Gel extraction

formerly known as "NucleoSpin® Extract II"

Features

PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• Two applications in one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)


Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 400 µL PCR reaction mixture < 400 mg TAE / TBE agarose gel
Fragment size	50 bp–approx. 20 kbp
Typical recovery	70–95%
A260/A280	1.8–1.9
Elution volume	15–30 µL
Preparation time	10 min/6 preps
Binding capacity	25 µg

Applications

• Purification of PCR products • Extraction of DNA from agarose gels
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)
• Support protocol for DNA extraction from polyacrylamide gels • Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”) • Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis