NucleoSpin® Gel and PCR Clean-up	PCR clean-up / Gel extraction
formerly known as "NucleoSpin® Extract II"
Features
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume
• Two applications one kit, one buffer with optimal performance for both applications • High recoveries for fragments down to 50 bp • Minimized elution volume: 15 μL → highly concentrated DNA • Binding buffer with pH indicator • Separate buffers for single stranded DNA or SDS containing samples available • Suitable for all gel buffer systems (e.g., TAE, TBE)

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 400 µL PCR reaction mixture < 400 mg TAE / TBE agarose gel
Fragment size	50 bp–approx. 20 kbp
Typical recovery	70–95%
A260/A280	1.8–1.9
Elution volume	15–30 µL
Preparation time	10 min/6 preps
Binding capacity	25 µg
Applications
• Purification of PCR products • Extraction of DNA from agarose gels
• Support protocol for concentration and desalination of reaction mixtures • Support protocol for clean-up of single stranded DNA (Binding Buffer NTC required, not included in the kit,    see “Ordering information”) • Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the    kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis