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NucleoBond® Finalizer / Finalizer Large
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Transfection-grade plasmid DNA
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| Features |
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| Desalting tools for speeding up the time-consuming precipitation step after anion-exchange plasmid purification |
• Eliminates centrifugation steps for precipitation → time saving from >1 h to only 5 min
• Two sizes available, to be combined with Midi and Maxi preparations
• Highly concentrated plasmid DNA depending on elution volume
• No loss of DNA pellets or incomplete solubilization of hardly visible precipitates |
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NucleoBond® Finalizer
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NucleoBond® Finalizer Large
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| Format |
Syringe filter
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| Sample material |
Plasmid and cosmid DNA eluates obtained by
anion-exchange chromatography
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| Vector size |
2–50 kbp
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2–50 kbp
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| Typical recovery |
40–90%
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40–90%
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Typical concentration
(elution volume dependent) |
0.1–3 µg/µL
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0.1–3 µg/µL
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| Elution volume |
> 200 µ/L
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> 400 µL
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| Preparation time |
5 min
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5 min
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| Residual chloride concentration |
< 0.3 µg/µL
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< 0.3 µg/µL
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| Binding capacity |
500 µg
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2000 µg
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| To be combined with |
NucleoBond® PC 100
NucleoBond® PC 500
NucleoBond® PC 500 EF
NucleoBond® Xtra Midi
NucleoBond® Xtra Midi EF
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NucleoBond® Xtra Maxi
NucleoBond® Xtra Maxi EF
NucleoBond® PC 2000
NucleoBond® PC 2000 EF
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| Applications |
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• Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange
chromatography
• Typical downstream applications: transfection, in-vitro transcription, automated or manual sequencing,
cloning, hybridization, and PCR
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Ordering information
| Product |
Preps
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Specification |
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| NucleoBond® Finalizer |
20
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20 Finalizers, 2 x 30 mL and 2 x 1 mL syringes |
740519.20 |
| NucleoBond® Finalizer Plus |
20
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20 Finalizers, 20 x 30 mL and
20 x 1 mL syringes |
740520.20 |
| NucleoBond® Finalizer Large |
20
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20 Finalizers Large, 2 x 30 mL and
2 x 1 mL syringes |
740418.20 |
| NucleoBond® Finalizer Large Plus |
20
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20 Finalizers Large, 20 x 30 mL and
20 x 1 mL syringes |
740419.20 |
For ordering click here
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