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NucleoBond­® Finalizer

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Mini note NucleoBond®
Finalizer

  
     

NucleoBond® Finalizer / Finalizer Large

Transfection-grade plasmid DNA

Features 
Desalting tools for speeding up the time-consuming precipitation step after anion-exchange plasmid purification

• Eliminates centrifugation steps for precipitation – time saving from >1 h to only 5

 
min

• Two sizes available, to be combined with Midi and Maxi preparations
• Highly concentrated plasmid DNA
• No loss of DNA pellets or incomplete solubilization of hardly visible precipitates


NucleoBond® Finalizer

NucleoBond® Finalizer Large

Format

Syringe filter

Sample material

Plasmid and cosmid DNA eluates obtained by
anion-exchange chromatography

Vector size

2–50 kbp

2–50 kbp

Typical recovery

40–90%

40–90%

Typical concentration
(elution volume dependent)

0.1–3 µg/µL

0.1–3 µg/µL

Elution volume

> 200 µ/L

> 400 µL

Preparation time

5 min

5 min

Residual chloride concentration

< 0.3 µg/µL

< 0.3 µg/µL

Binding capacity

500 µg

2000 µg

To be combined with

NucleoBond® PC 100
NucleoBond® PC 500
NucleoBond® PC 500 EF
NucleoBond® Xtra Midi
NucleoBond® Xtra Midi EF

NucleoBond® Xtra Maxi
NucleoBond® Xtra Maxi EF
NucleoBond® PC 2000
NucleoBond® PC 2000 EF

Applications

Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange
   chromatography
• Typical downstream applications: transfection, in-vitro transcription, automated or manual sequencing,
   cloning, hybridization, and PCR   

  
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Ordering information

Product

Preps

Specification REF
NucleoBond® Finalizer

20

20 Finalizers, 2 x 30 mL and 2 x 1 mL syringes 740519.20
NucleoBond® Finalizer Plus

20

20 Finalizers, 20 x 30 mL and
20 x 1 mL syringes
740520.20
NucleoBond® Finalizer Large

20

20 Finalizers Large, 2 x 30 mL and
2 x 1 mL syringes
740418.20
NucleoBond® Finalizer Large Plus

20

20 Finalizers Large, 20 x 30 mL and
20 x 1 mL syringes
740419.20

For ordering click here

  
   
  
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