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Protino® Ni-TED Packed Columns
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Purification of His-tag proteins
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High specificity → highest purity of His-tag proteins
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| Features |
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Protino® Ni-TED, the purest alternative for His-tag protein purification
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• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding
site provides highest selectivity and purity (also see Technologies)
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins
compared to other common IMAC matrices
• Dry resin → storage at room temperature
Protino® Ni-TED Packed Columns
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included
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Protino® Ni-TED
150 Packed Columns
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Protino® Ni-TED
1000 Packed Columns
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Protino® Ni-TED
2000 Packed Columns
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| Technology |
IMAC (immobilized metal ion affinity chromatography)
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| Chelating ligand |
TED (tris-carboxymethyl ethylene diamine)
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| Format |
Mini
gravity flow columns
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Midi
gravity flow columns
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Maxi
gravity flow columns
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| Matrix |
Macroporous silica
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| Physical form |
Dry matrix, precharged with Ni2+
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Amount of resin
per column
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40 mg
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250 mg
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500 mg
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| Bed volume |
80 µL
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500 µL
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1000 µL
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| Binding capacity* |
400 µg
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2.5 mg
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5 mg
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| * Binding capacity will vary for each polyhistidin-tagged protein |
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Applications
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| • Purification of polyhistidine-tagged proteins |
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Ordering information
| Product |
Preps
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Specification |
REF |
| Protino® Ni-TED 150 Packed Columns |
10
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Protino® Ni-TED 150 Packed Columns, buffers |
745100.10 |
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50
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as above |
745100.50 |
| Protino® Ni-TED 1000 Packed Columns |
5
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Protino® Ni-TED 1000 Packed Columns, buffers |
745110.5 |
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50
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as above |
745110.50 |
| Protino® Ni-TED 2000 Packed Columns |
5
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Protino® Ni-TED 2000 Packed Columns, buffers |
745120.5 |
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25
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as above |
745120.25 |
For ordering click here
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