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NucleoSpin® Plasmid /
Plasmid (NoLid)


Supplementary protocols
Supplementary protocols plasmid DNA

  
     
NucleoSpin® Plasmid Sequencing-grade plasmid DNA
NucleoSpin® Plasmid (NoLid)
Features
For rapid small scale preparation high-purity plasmid DNA

NucleoSpin Plasmid Columns• Fast procedure – 18 Minipreps in 25 min
• High capacity – up to 50 μg of plasmid DNA
• Chaotropic salt wash buffer – extra pure DNA


Also available for QIAcube® (please see "Ordering information" and contact Technical Service
   at
techbio@mn-net.com for more information).

Technology Silica-membrane technology
Format Mini spin columns
Sample material 2–10 mL E. coli culture
Vector size < 15 kbp
Typical yield 25–45 µg
A260/A280 1.80–1.85
Elution volume 50 µL
Preparation time 25 min/6 preps
Binding capacity 50 µg
Applications
• High-copy plasmids from E. coli 
• Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required, see "Ordering information"
• Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction
   mixtures
• Supplementary protocol for M13 DNA available on request, please contact Technical Service
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® Plasmid

10

NucleoSpin® Plasmid Columns, Collection Tubes (2 mL), buffers, RNase A 740588.10

50

as above 740588.50

250

as above 740588.250
NucleoSpin® Plasmid (NoLid)

50

NucleoSpin® Plasmid Columns (NoLid), Collection Tubes (2 mL), buffers, RNase A 740499.50

250

as above 740499.250

240

Kit for use on QIAcube®*
NucleoSpin® Plasmid (NoLid) Columns, buffers, RNase A
740499.240C
Product accessories

Pack of

Specification REF
NucleoSpin® Plasmid Buffer Set

1

for the isolation of low-copy plasmids,
additional buffer set with 75 mL of buffers A1, A2, 100 mL of buffer A3, RNase A
740953

* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability.
MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences.

For ordering click here

  
   
  
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