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| NucleoSpin® Plasmid |
Sequencing-grade plasmid DNA
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| NucleoSpin® Plasmid (NoLid) |
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| Features |
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| For rapid small scale preparation of up to 40 μg of high-purity plasmid DNA |
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 • Fast procedure: 18 Mini preps in 25 min
• High capacity: up to 40 μg of plasmid DNA
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
1–5 mL E. coli culture (standard protocol)
5–10 mL E. coli culture* |
| Vector size |
< 15 kbp |
| Typical yield |
< 25 µg (from 1–5 mL E. coli culture)
< 40 µg (from 5–10 mL E. coli culture) |
| A260/A280 |
1.80–1.85 |
| Elution volume |
50 µL |
| Preparation time |
25 min/18 preps |
| Binding capacity |
60 µg |
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| Applications |
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• High-copy plasmids from E. coli
• Support protocol for low-copy plasmids, P1 constructs, or cosmids*
• Support protocols for plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction
mixtures
• Support protocol for M13 DNA available on request, please contact Technical Service
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
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| * Increased buffer volumes required, see "Ordering information" |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® Plasmid |
10
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NucleoSpin® Plasmid Columns, Collection Tubes (2 mL), buffers, RNase A |
740588.10 |
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50
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as above |
740588.50 |
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250
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as above |
740588.250 |
| NucleoSpin® Plasmid (NoLid) |
50
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NucleoSpin® Plasmid Columns (NoLid), Collection Tubes (2 mL), buffers, RNase A |
740499.50 |
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250
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as above |
740499.250 |
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| Product accessories |
Pack of
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Specification |
REF |
NucleoSpin® Plasmid Buffer Set
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1
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for the isolation of low-copy plasmids, buffers A1, A2, A3, RNase A,
sufficient for 300 preps |
740953 |
For ordering click here
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