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| NucleoBond® BAC 100 |
Transfection-grade plasmid DNA
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| Features |
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| The anion exchanger for purification of P1, BACs, PACs, and other large constructs |
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• Suitable for constructs up to 300 kbp
• Optimized lysis buffer volumes for large bacterial cultures
• NucleoBond® Folded Filters included for gentle lysate clarification without shearing of large
constructs
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| Technology |
Anion-exchange chromatography |
| Format |
Gravity-flow columns |
| Lysate clarification |
Folded filters |
| Sample material |
100–500 mL E.coli culture |
| Vector size |
< 300 kbp |
| Typical yield |
10–100 µg |
| A260/A280 |
1.80–1.95 |
| Preparation time |
120 min/2–4 preps |
| Binding capacity |
100 µg |
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| Applications |
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• Purification of P1, BACs, PACs, and other large constructs from E. coli
• Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoBond® BAC 100 |
10
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NucleoBond® BAC 100 Columns, buffers,
RNase A, NucleoBond® Folded Filters |
740579 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoBond® Rack Large |
1
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for use with NucleoBond® AX 100, 500, 2000,
10000, BAC 100, and Xtra Midi Columns
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740563 |
For ordering click here
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