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| NucleoBond® PC EF |
Endotoxin-free plasmid DNA
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| Features |
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| The classic anion exchanger for purification of endotoxin-free plasmid DNA |
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• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
• Patented endotoxin removal by additional washing step – no extra ice incubation necessary
• Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required,
no shearing forces
• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for
plasmid precipitation
• Transfection-grade plasmid DNA due to patented anion-exchange technology
• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns
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NucleoBond®
PC 500 EF
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NucleoBond®
PC 2000 EF
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NucleoBond®
PC 10000 EF
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NucleoBond®
PC Prep 100
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| Technology |
Anion-exchange chromatography
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| Format |
Maxi
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Mega
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Giga
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Preparative
Scale
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Gravity-flow
columns
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Gravity-flow
columns
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Gravity-flow
columns
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Compatible with
e.g., HPLC, FPLC
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| Lysate clarification |
Folded filters
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Bottle top
filters
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Bottle top
filters
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Folded filters,
sieving fabric
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| Sample material |
30–150 mL
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150–500 mL
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500–2000 mL
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5–20 L
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Vector size
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< 300 kbp
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< 300 kbp
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< 300 kbp
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< 300 kbp
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| Typical yield |
450–520 µg
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1700–
2000 µg
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5000–
9000 µg
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80–100 mg
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| A260/A280 |
1.80–1.95
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1.80–1.95
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1.80–1.95
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1.80–1.95
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| Endotoxin level |
< 0.1 EU/µg
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< 0.1 EU/µg
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< 0.1 EU/µg
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< 0.1 EU/µg
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| Preparation time |
100 min/
2 preps
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150 min/
2 preps
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180 min/
2 preps
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20 h/
prep
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| Binding capacity |
500 µg
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2000 µg
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10000 µg
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100 mg
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| Applications |
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• High-copy plasmids from E. coli
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoBond® PC 500 EF |
10
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NucleoBond® AX 500 Columns, buffers EF, RNase A, NucleoBond® Folded Filters XL |
740550 |
| NucleoBond® PC 2000 EF |
5
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NucleoBond® AX 2000 Columns, buffers EF, RNase A, NucleoBond® Bottle Top Filters |
740549 |
| NucleoBond® PC 10000 EF |
5
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NucleoBond® AX 10000 Columns, buffers EF, RNase A, NucleoBond® Bottle Top Filters |
740548 |
| NucleoBond® PC Prep 100 |
1
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1 NucleoBond® AX Prep 100 Column,
3 NucleoBond® AX 100 Columns, buffers EF, RNase A, Sieving Fabric, NucleoBond® Folded Filters |
740594 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoBond® Large Rack |
1
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for use with NucleoBond® AX 100, 500, 2000, 10000, and Xtra Midi Columns |
740563 |
For ordering click here
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