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| NucleoBond® PC |
Transfection-grade plasmid DNA
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| Features |
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| The classic anion exchanger for purification of transfection-grade plasmid DNA |
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 • Fast and easy clarification of lysates with NucleoBond® Folded Filters, no centrifugation required,
no shearing forces
• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for
plasmid precipitation
• Transfection-grade plasmid DNA due to patented anion-exchange technology
• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX colums
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NucleoBond®
PC 20
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NucleoBond®
PC 100
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NucleoBond®
PC 500
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NucleoBond®
PC 2 000
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NucleoBond®
PC 10 000
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| Technology |
Anion-exchange chromatography
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| Format |
Mini
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Midi
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Maxi
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Mega
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Giga
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Gravity-flow columns
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| Lysate clarification |
Centrifugation
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Folded filters
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Folded filters
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Folded filters
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Folded filters
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| Sample material |
1–5 mL (HC*)
3–10 mL (LC**)
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5–30 mL (HC*)
10–100 mL (LC**)
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30–150 mL (HC*)
100–500 mL (LC**)
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150–500 mL
(HC*) |
500–2000 mL
(HC*) |
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Vector size
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< 300 kbp
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< 300 kbp
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< 300 kbp
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< 300 kbp
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< 300 kbp
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| Typical yield |
3–20 µg
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20–100 µg
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450–520 µg
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1700–
2000 µg
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5000–
9000 µg
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| A260/280 |
1.80–1.95
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1.80–1.95
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1.80–1.95
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1.80–1.95
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1.80–1.95
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| Preparation time |
60 min/
4–6 preps
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65 min/
4–6 preps
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80–90 min/
4 preps
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90–120 min/
4–6 preps
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120–150 min/
2 preps
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| Binding capacity |
20 µg
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100 µg
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500 µg
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2000 µg
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10000 µg
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* High-copy plasmids
** Low-copy plasmids
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| Applications |
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• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli*** |
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| *** Increased buffer volumes required, see "Ordering information" |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoBond® PC kits for purification of plasmids and cosmids |
| NucleoBond® PC 20 |
20
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NucleoBond® AX 20 Columns, buffers, RNase A |
740571 |
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100
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as above |
740571.100 |
| NucleoBond® PC 100 |
20
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NucleoBond® AX 100 Columns, buffers, RNase A, NucleoBond® Folded Filters |
740573 |
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100
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as above
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740573.100 |
| NucleoBond® PC 500 |
10
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NucleoBond® AX 500 Columns, buffers, RNase A, NucleoBond® Folded Filters XL |
740574 |
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25
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as above |
740574.25 |
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50
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as above |
740574.50 |
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100
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as above |
740574.100 |
| NucleoBond® PC 2000 |
5
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NucleoBond® AX 2000 Columns, buffers, RNase A, NucleoBond® Folded Filters XL |
740576 |
| NucleoBond® PC 10000 |
5
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NucleoBond® AX 10000 Columns, buffers, RNase A, NucleoBond® Folded Filters (Type 1, Type 2) |
740593 |
| NucleoBond® AX columns |
| NucleoBond® AX 20 |
20
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NucleoBond® AX 20 Columns |
740511 |
| NucleoBond® AX 100 |
20
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NucleoBond® AX 100 Columns |
740521 |
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100
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as above |
740521.100 |
| NucleoBond® AX 500 |
10
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NucleoBond® AX 500 Columns
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740531 |
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50
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as above |
740531.50 |
| NucleoBond® AX 2000 |
10
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NucleoBond® AX 2000 Columns
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740525 |
| NucleoBond® AX 10000 |
5
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NucleoBond® AX 10000 Columns
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740534 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoBond® accessories |
| NucleoBond® Small Rack |
1
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for use with NucleoBond® AX 20 Columns |
740562 |
| NucleoBond® Large Rack |
1
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for use with NucleoBond® AX 100, 500, 2000, 10000, BAC 100, and Xtra Midi Columns |
740563 |
| NucleoBond® buffers |
| NucleoBond® Buffer Set I |
1
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for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® PC kits, sufficient for 10 AX 500 Columns (Maxi preps), 20 AX 100 Columns (Midi preps), set incl. RNase A |
740601 |
For ordering click here
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